Phospho-SF3B1-T313 Rabbit pAb for WB, ELISA - O75533

Core Product Specifications and Parameters

Biological Background: SF3B1 Function and Localization

• SF3B1 is a core component of the 17S U2 snRNP complex, essential for pre-mRNA splicing by recognizing the branch site and promoting branch-site adenosine selection. Related references: PMID:32494006, PMID:34822310, PMID:36104565
• It belongs to the SF3B subcomplex, required for stable U2 snRNP binding to the branchpoint sequence during 'A' complex assembly. Related references: PMID:12234937, PMID:36104565
• SF3B1 may also participate in the early 'E' complex assembly. Related references: PMID:10882114
• It functions as a component of the minor spliceosome, splicing U12-type introns. Related references: PMID:15146077, PMID:33509932
• Subcellularly, SF3B1 localizes to the nucleus and nuclear speckles.
• The protein undergoes multiple post-translational modifications, including phosphorylation, acetylation, citrullination, and ubiquitination.
• It may also contribute to the processing of microRNAs, such as pri-miR-17-92.

Experimental Guidance and Technical Tips

• Since this antibody recognizes phosphorylation at T313, include phosphatase inhibitors during sample preparation to preserve the phospho-epitope.
• For western blotting, the predicted molecular weight of SF3B1 is approximately 146 kDa; verify detection in your specific sample system.
• The antibody has been tested in WB and ELISA and shows cross-reactivity with human, mouse, and rat; validate in the relevant species and tissue context.
• Consider using the antibody in phospho-peptide competition assays to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, offering validated antibodies, antigens, and ancillary reagents to support diagnostic development and life science research. This phospho-SF3B1 (T313) polyclonal antibody exemplifies our commitment to delivering high-quality tools for spliceosome and RNA processing studies. Whether you need rare-target antibodies, bulk production, or tailored matched-pair sourcing, CamelBio can help streamline your procurement from concept to clinic.