Anti-DMT1/SLC11A2 Monoclonal Antibody for WB - P49281

Core Product Specifications and Parameters

Biological Background: DMT1/SLC11A2 Function and Localization

• DMT1/SLC11A2, also known as natural resistance-associated macrophage protein 2, divalent cation transporter 1, or divalent metal transporter 1 (DMT-1), is a member of the solute carrier family 11.
• It functions as a proton-coupled metal ion symporter that selectively transports divalent metal cations with affinities in the order Cd²⁺ > Fe²⁺ > Co²⁺, Mn²⁺ >> Zn²⁺, Ni²⁺, VO²⁺. Related references: PMID:17109629, PMID:17293870, PMID:22736759
• DMT1/SLC11A2 is essential for iron homeostasis, mediating intestinal absorption of dietary Fe²⁺ and transferrin-associated endosomal Fe²⁺ transport in erythroid precursors and other cells.
• The protein facilitates Fe²⁺ and Mn²⁺ ion entry into mitochondria, contributing to mitochondrial heme synthesis, iron-sulfur cluster biogenesis, and antioxidant defense. Related references: PMID:24448823
• It localizes to multiple cellular membranes including early and late endosomes, lysosomes, the apical cell membrane, cell membrane, mitochondrial outer membrane, Golgi apparatus, and recycling endosome membrane.
• Ubiquitously expressed across tissues, with notable expression in erythroid progenitors.
• Post-translational modifications include glycosylation and phosphorylation, as indicated by UniProt keywords.
• The gene undergoes alternative splicing; disease-associated variants have been identified.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 300-400 of human DMT1/SLC11A2 (NP_000608.1). This region may provide reactivity against multiple isoforms and across species, consistent with the observed human and mouse cross-reactivity.
• For Western blot (WB), DMT1/SLC11A2 is a transmembrane protein; consider using appropriate lysis and sample preparation protocols to efficiently extract membrane proteins. The predicted molecular weights range from 61 to 65 kDa, which may represent different glycosylation or splice isoforms.
• For immunofluorescence/immunocytochemistry (IF/ICC), localization to endosomal and plasma membrane compartments can be expected; permeabilization and fixation conditions should be optimized for membrane antigens.
• The rabbit monoclonal format typically ensures high specificity and lot-to-lot consistency, suitable for quantitative assays like ELISA and IP.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and laboratories from concept to clinical application. This anti-DMT1/SLC11A2 antibody is offered through CamelBio's extensive portfolio of validated monoclonal and polyclonal antibodies targeting metal ion transporters and iron metabolism pathways. Beyond individual antibodies, CamelBio provides integrated supply solutions including optimized antibody pairs, bulk reagents, and custom raw-material sourcing for rare and specialized targets.