Anti-Desmin Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P17661

Core Product Specifications and Parameters

Biological Background: Desmin Function and Localization

• Desmin is a muscle-specific type III intermediate filament protein encoded by the DES gene, crucial for maintaining sarcomere structure and myofibril integrity.
• It interconnects Z-disks and myofibrils, linking them to the sarcolemmal cytoskeleton, nuclei, and mitochondria, providing mechanical strength during muscle contraction. Related references: PMID:25358400
• In adult striated muscle, desmin forms a fibrous network connecting myofibrils to each other and to the plasma membrane from the Z-line periphery. Related references: PMID:24200904, PMID:25394388, PMID:26724190
• Desmin acts as a sarcomeric microtubule-anchoring protein, binding detyrosinated tubulin-alpha to stabilize microtubules and resist contraction forces (By similarity).
• It supports nuclear membrane integrity by anchoring at cell tips and the nuclear envelope, maintaining intracellular mechanical forces (By similarity).
• Desmin contributes to transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells and adult cardiac stem cells, influencing cardiomyogenesis (By similarity).
• Subcellular localization includes cytoplasm, myofibril sarcomere Z line, sarcolemma, nucleus, and cell tip, reflecting its structural and regulatory roles.
• Post-translational modifications include phosphorylation, methylation, ADP-ribosylation, and ubiquitination, while disease associations cover cardiomyopathy, desmin-related myopathy, limb-girdle muscular dystrophy, and myofibrillar myopathy.

Experimental Guidance and Technical Tips

• The immunogen (synthetic peptide within amino acids 371-470) targets the C-terminal region of human Desmin; therefore, the antibody is expected to recognize full-length Desmin and its isoforms.
• For Western blotting, a band at ~54 kDa is expected in human, mouse, or rat muscle tissue lysates; validate detection of post-translational modifications if relevant.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), use frozen or paraffin-embedded muscle sections; antigen retrieval may be considered for formalin-fixed tissues.
• For ELISA, the antibody can be used as a capture or detection reagent; optimization with recombinant Desmin or peptide coating is recommended.
• Cross-reactivity with human, mouse, and rat is confirmed; for other species, check sequence homology of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This Anti-Desmin antibody is an example of our muscle biology and cardiovascular disease research portfolio. We support diagnostic manufacturers and labs with reliable supply chains from concept to clinic.