Anti-Alcohol Dehydrogenase 1A Monoclonal Antibody for WB, ELISA - P07327

Core Product Specifications and Parameters

Biological Background: Alcohol Dehydrogenase 1A (ADH1A) Function and Localization

• Alcohol dehydrogenase 1A (ADH1A) catalyzes the oxidation of primary and secondary alcohols, using NAD+ as a cofactor; ethanol is a very poor substrate. Related references: PMID:2738060
• ADH1A is a member of the alcohol dehydrogenase family and contains zinc-binding sites essential for its enzymatic activity.
• The protein is localized in the cytoplasm.
• Post-translational modifications of ADH1A include acetylation and phosphorylation, which may regulate its function.
• UniProt keywords associated with ADH1A include Metal-binding, NAD, Oxidoreductase, and Zinc, and it is part of the reference proteome.

Experimental Guidance and Technical Tips

• For western blotting, a band around 40 kDa is expected; validate the antibody in the relevant sample system.
• For ELISA, consider standard direct or sandwich formats with this monoclonal antibody as detection reagent, and optimize coating and detection conditions.
• The antibody cross-reacts with mouse ADH1; verify specificity and sensitivity in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies such as this ADH1A antibody. From bulk reagents and optimized antibody pairs to technical consulting, we support your alcohol metabolism research and assay development from concept to clinic.