Anti-SLC25A12 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, IP, ELISA - O75746

Core Product Specifications and Parameters

Biological Background: SLC25A12 Function and Localization

• SLC25A12, also known as Araceli hiperlarga (Aralar/Aralar1) or mitochondrial aspartate glutamate carrier 1 (AGC1), is a member of the solute carrier family 25 and functions as a mitochondrial electrogenic aspartate/glutamate antiporter.
• As a core component of the malate-aspartate shuttle, SLC25A12 facilitates the efflux of aspartate in exchange for glutamate and a proton across the mitochondrial inner membrane, linking cytosolic and mitochondrial metabolism. Related references: PMID:11566871 PMID:19641205 PMID:24515575
• This carrier also mediates the uptake of L-cysteinesulfinate (3-sulfino-L-alanine) into mitochondria in exchange for L-glutamate and a proton, and can exchange cysteinesulfinate with aspartate without proton translocation. Related references: PMID:11566871
• SLC25A12 lacks transport activity towards L-glutamine or gamma-aminobutyric acid (GABA), demonstrating substrate specificity. Related references: PMID:38945283
• The protein localizes to the mitochondrial inner membrane and is expressed predominantly in heart and skeletal muscle, with lower levels in brain and kidney.
• SLC25A12 is implicated in disease (alternative splicing and disease variant keywords), and its sequence contains EF-hand calcium-binding domains, suggesting regulation by calcium.
• Post-translational modifications include acetylation, and the protein is a reference proteome member with a calculated molecular weight of ~75 kDa.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen suggests that this rabbit monoclonal antibody likely recognizes a conformational or linear epitope within the SLC25A12 sequence; perform epitope mapping if precise binding site is required.
• For western blot (WB), the predicted molecular weight of SLC25A12 is approximately 75 kDa; validate detection in relevant tissue lysates (e.g., human, mouse, or rat heart or skeletal muscle) and consider loading controls.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), expect mitochondrial staining; include organelle markers to confirm subcellular localization.
• This antibody is suitable for immunoprecipitation (IP) and ELISA; optimize experimental conditions (e.g., antibody concentration, incubation times) for each assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing access to a comprehensive portfolio of antibodies, antigens, and ancillary reagents. This anti-SLC25A12 monoclonal antibody targets the mitochondrial aspartate/glutamate carrier, supporting research in metabolic shuttles, mitochondrial dysfunction, and related disorders. CamelBio offers validated antibody pairs, bulk supplies, and custom sourcing solutions to streamline your diagnostic and research workflows.