Anti-SLC6A2 Rabbit Monoclonal Antibody for WB, ELISA - P23975

Core Product Specifications and Parameters

Biological Background: SLC6A2 Function and Localization

• SLC6A2, also known as the norepinephrine transporter (NET) or solute carrier family 6 member 2, is encoded by the SLC6A2 gene (alternative names: NAT1, NET1, SLC6A5). It undergoes alternative splicing, generating isoforms with potentially distinct functions.
• The protein mediates sodium- and chloride-dependent transport of norepinephrine, the primary neurotransmitter in the sympathetic nervous system, and is responsible for its reuptake and clearance from the synaptic cleft, thereby regulating neurotransmitter homeostasis. Related references: PMID:2008212, PMID:8125921, PMID:38750358
• In addition to norepinephrine, SLC6A2 can also transport dopamine in a sodium- and chloride-dependent manner, broadening its role in monoamine signaling. Related references: PMID:11093780, PMID:8125921, PMID:39395208
• Subcellularly, SLC6A2 is localized to the cell membrane, particularly at presynaptic axon terminals and synaptosomes, where it actively reclaims released neurotransmitters.
• The protein is characterized by the presence of disulfide bonds, glycosylation sites, lipidation (palmitoylation), and metal-binding motifs, which may contribute to its structural stability and functional regulation.
• SLC6A2 is a member of the neurotransmitter:sodium symporter family and contains multiple transmembrane helices characteristic of membrane transporters; it is annotated with keywords including alternative splicing, disease variant, glycoprotein, and synapse.

Experimental Guidance and Technical Tips

• The immunogen (N-terminal amino acids 1–70) may target an accessible epitope; consider confirming antibody specificity using SLC6A2-overexpressing cell models or tissue samples where the transporter is highly expressed.
• For Western blot (WB) analysis, the predicted molecular weight is ~69 kDa (UniProt: 69332 Da); run an appropriate molecular weight marker and consider potential post‑translational modifications that may shift the observed band.
• In ELISA applications, the antibody can be used for direct or sandwich formats; pairing with a polyclonal antibody or another monoclonal clone may improve sensitivity, and it is advisable to validate performance in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

As a premier IVD raw‑material partner, CamelBio streamlines your development by offering validated antibody pairs, bulk monoclonal antibodies, and ancillary reagents alongside expert technical consultation. For neurotransmitter transporter research and diagnostic projects, we supply high‑quality anti‑SLC6A2 antibodies and can support the sourcing of rare target raw materials to accelerate your assay development from concept to clinic.