Anti-BDNF Monoclonal Antibody (Mouse) for WB, ELISA - P23560

Core Product Specifications and Parameters

Biological Background: BDNF Function and Localization

• BDNF (Brain-derived neurotrophic factor), also known as abrineurin, is a secreted neurotrophic factor of the nerve growth factor family, encoded by the BDNF gene on human chromosome 11.
• BDNF is initially synthesized as a precursor (proBDNF) that is cleaved to yield the mature 14 kDa protein; the immunogen spans the pro-region and mature domain (aa 20–247).
• BDNF plays a critical role in neuronal survival, differentiation, axonal and dendritic growth, and synaptic plasticity by activating the NTRK2 receptor, with a key signaling role in long-term potentiation (LTP) and depression (LTD) (PubMed:11152678). Related references: PMID:11152678
• BDNF also engages the heterodimeric receptor formed by NGFR and SORCS2, triggering signaling cascades that influence synaptic plasticity, LTD, and neuronal apoptosis, as well as promoting growth cone collapse (PubMed:24908487, PubMed:29909994). Related references: PMID:24908487 PMID:29909994
• Predominantly localized to the secretory pathway, BDNF is highly expressed in the hippocampus, amygdala, cerebral cortex, and cerebellum, with detectable levels in blood plasma and saliva.
• Structural features include a signal peptide, a disulfide-bonded knot motif typical of growth factors, and multiple N-glycosylation sites.

Experimental Guidance and Technical Tips

• When performing Western blot, expect bands around 28 kDa (proBDNF) and potentially 14 kDa (mature BDNF) depending on sample processing; validate with recombinant protein controls.
• For ELISA, titrate the antibody against recombinant human BDNF (aa 20–247) to optimize detecting both pro- and mature forms; consider testing cross-reactivity with mouse and rat orthologs.
• This monoclonal antibody is raised against an immunogen spanning the pro-region, so it should recognize proBDNF robustly; detection of mature BDNF may require optimized conditions.
• Validate antibody specificity in your target tissue or cell line, as BDNF expression varies; use knockdown or knockout controls where possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For BDNF-related neuroscience and biomarker studies, we offer validated monoclonal and polyclonal antibodies, optimized ELISA antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to accelerate your BDNF assay development with reliable supply and expert support.