Anti-SLC4A1 Polyclonal Antibody for WB, IF-P, ELISA - P02730

Core Product Specifications and Parameters

Biological Background: SLC4A1 Function and Localization

• SLC4A1 encodes the Band 3 anion transport protein (AE1), a multifunctional protein that serves as an electroneutral chloride-bicarbonate exchanger and a key structural component of the erythrocyte plasma membrane. Related references: PMID:10926824, PMID:14734552, PMID:1538405
• In erythrocytes, the cytoplasmic N-terminal domain interacts with ankyrin-1, cytoskeletal proteins, glycolytic enzymes, and hemoglobin, contributing to membrane flexibility, stability, and the biconcave disc shape. Related references: PMID:1538405, PMID:20151848, PMID:35835865
• In the kidney, SLC4A1 is localized to the basolateral membrane of alpha-intercalated cells in the collecting duct, where it mediates chloride-bicarbonate exchange essential for systemic acid-base balance and urine acidification. Related references: PMID:10926824, PMID:14734552, PMID:16227998
• SLC4A1 acts as a receptor for Plasmodium falciparum merozoite surface proteins MSP1 and MSP9, facilitating parasite invasion of erythrocytes. Related references: PMID:14630931, PMID:12692305
• The protein belongs to the solute carrier family 4 (anion exchangers) and is subject to alternative splicing, producing isoforms with distinct N-terminal cytoplasmic domains; the canonical isoform is a glycoprotein with 12–14 transmembrane spans.
• Post-translational modifications include phosphorylation, acetylation, palmitoylation, and glycosylation; it is also a lipoprotein, anchored in the membrane via palmitate.
• Mutations in SLC4A1 are associated with hereditary hemolytic anemias, including hereditary spherocytosis type 4 (SPH4), Southeast Asian ovalocytosis (SAO), and distal renal tubular acidosis (dRTA) with/without anemia.
• High expression is detected in erythrocytes and kidney, with additional tissue-specific isoforms reported.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal cytoplasmic domain (amino acids 1–353), so the antibody likely recognizes epitopes in this region. Consider verifying epitope specificity using truncated protein fragments or blocking peptides.
• For Western blotting, SLC4A1 has a predicted molecular weight of 102 kDa; ensure appropriate gel percentage and transfer conditions, and validate in a relevant sample system (e.g., erythrocyte lysates).
• For immunofluorescence (IF-P), permeabilization is recommended to access the cytoplasmic epitope. Co-staining with membrane markers may help confirm plasma membrane localization.
• Cross-reactivity with mouse and rat allows for testing in rodent models; however, validate specificity in tissue/cell types known to express SLC4A1.
• ELISA can be employed for quantitative detection; consider using recombinant protein standards for calibration.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop IVD raw material sourcing partner, CamelBio supports diagnostic manufacturers and research institutes with high-quality antibodies, antigen pairs, and ancillary reagents. This anti-SLC4A1 antibody enables studies on erythrocyte membrane disorders, renal physiology, and malaria invasion mechanisms. We offer flexible procurement of validated antibody pairs, bulk monoclonal/polyclonal antibodies, and custom sourcing for rare targets to streamline your assay development from concept to clinic.