Anti-DMRT1 Polyclonal Antibody for WB, ELISA - Q9Y5R6

Core Product Specifications and Parameters

Biological Background: DMRT1 Function and Localization

• Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation.
• Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm.
• Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1.
• Plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes.
• May act as a tumor suppressor and may also play a minor role in oogenesis (By similarity).
• Testis-specific expression, also detected in prostate cancer at protein level; localized to the nucleus.
• Contains a DM domain for DNA binding and a zinc-finger motif, classified as a developmental protein involved in sexual differentiation and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 134–373 of human DMRT1, covering the DM domain and C-terminal regions; consider this when analyzing detection of isoforms or homologs.
• For WB, a band around 39kDa is expected in human testis lysates; validate in the relevant sample system with appropriate controls.
• For ELISA, consider using the recombinant protein as a coating antigen for antibody titer and specificity assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material sourcing solutions, supporting diagnostic manufacturers and research institutions with a wide range of antibodies and reagents. This anti-DMRT1 polyclonal antibody is ideal for reproductive biology, developmental research, and oncology studies. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development and clinical translation.