Anti-ALDH1A2 Monoclonal Antibody for WB, IHC-P, ELISA - O94788

Core Product Specifications and Parameters

Biological Background: ALDH1A2 Function and Localization

• Alternative splicing produces multiple transcript variants, and the encoded protein belongs to the aldehyde dehydrogenase family.
• Catalyzes the NAD-dependent oxidation of retinaldehyde to retinoic acid, playing a key role in retinoic acid signaling for transcriptional regulation and meiosis initiation. Related references: PMID:29240402 PMID:33565183
• Exhibits strong substrate preference for all-trans-retinal and all-trans-13,14-dihydroretinal, with minimal activity toward short-chain aldehydes.
• Localizes predominantly to the cytoplasm.
• Contains phosphorylation sites and has been associated with disease variants, indicating potential regulation and clinical relevance.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 57 kDa in lysates from ALDH1A2-expressing tissues (e.g., liver, testis). Include appropriate positive and negative controls.
• For immunohistochemistry, antigen retrieval methods should be optimized; recommended positive control tissues include testis or developing embryonic tissues.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with another clone may enhance sensitivity.
• This rabbit monoclonal antibody offers consistent lot-to-lot performance, minimizing experimental variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents, to support diagnostic assay development from R&D to clinical application. With expertise in sourcing rare targets like ALDH1A2, we provide one-stop access to high-quality monoclonal antibodies and custom technical services, ensuring seamless integration into your workflow.