Anti-DMT1/SLC11A2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P49281

Core Product Specifications and Parameters

Biological Background: DMT1/SLC11A2 Function and Localization

• DMT1/SLC11A2, also known as DCT1, DMT1, or NRAMP2, is encoded by the SLC11A2 gene and belongs to the solute carrier family 11.
• It functions as a proton-coupled metal ion symporter with a 1:1 proton:metal stoichiometry, transporting divalent cations with high affinity for Cd²⁺ and Fe²⁺, followed by Co²⁺, Mn²⁺, and with much lower affinity for Zn²⁺, Ni²⁺, and VO²⁺ (PubMed:17109629, PubMed:17293870, PubMed:22736759). Related references: PMID:17109629, PMID:17293870, PMID:22736759
• Essential for iron homeostasis, DMT1 mediates intestinal absorption of dietary Fe²⁺ and facilitates transferrin‑associated endosomal Fe²⁺ transport in erythroid precursors (By similarity). It also promotes mitochondrial Fe²⁺ and Mn²⁺ uptake, supporting heme synthesis and antioxidant defense (PubMed:24448823). Related references: PMID:24448823
• The protein localizes to multiple cellular membranes, including the plasma membrane, early, late, and recycling endosomes, lysosomes, mitochondrial outer membrane, Golgi apparatus, and extracellular vesicles.
• DMT1 is ubiquitously expressed, with notable expression in erythroid progenitors, consistent with its role in iron delivery for hemoglobin synthesis.
• Post‑translational modifications include glycosylation, phosphorylation, and ubiquitination, and alternative splicing generates multiple isoforms with distinct tissue distributions and subcellular localizations.

Experimental Guidance and Technical Tips

• The immunogen comprises the first 63 amino acids of the human DMT1/SLC11A2 N‑terminus (NP_001167597.1), which corresponds to the predicted intracellular domain. For applications requiring intracellular epitope access (e.g., IHC‑P), consider permeabilization steps.
• For Western blotting, a band corresponding to the predicted molecular weight of ~62 kDa should be observed under standard reducing conditions; minor bands may reflect tissue‑specific isoforms or glycosylation variants.
• When using this antibody for ELISA, the recombinant protein immunogen can serve as a positive control to assess reactivity and titer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop source for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support diagnostic and research applications. For iron transport and metal ion homeostasis targets like DMT1/SLC11A2, we supply rigorously characterized reagents to accelerate assay development. From concept to clinic, CamelBio bridges your raw material needs with reliable supply chain solutions.