Anti-DMTF1 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y222

Core Product Specifications and Parameters

Biological Background: DMTF1 Function and Localization

• DMTF1 (Cyclin-D-binding Myb-like transcription factor 1, also known as hDMP1) is a transcription factor that belongs to the Myb family. It exists in multiple isoforms due to alternative splicing, with isoform 1 being the predominant form in monocytes and macrophages, while isoform 2 is most strongly expressed in peripheral blood leukocytes and quiescent CD34+ cells.
• As a transcriptional activator, DMTF1 binds to the consensus DNA sequence 5'-CCCG[GT]ATGT-3' to activate the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest. This function underscores its role as a tumor suppressor.
• The protein contains Myb-like DNA-binding domains and localizes exclusively to the nucleus, consistent with its role in transcriptional regulation.
• DMTF1 is broadly expressed, with higher levels in spleen, testis, thymus, and various brain regions (amygdala, hippocampus, etc.), and lower levels in colonic mucosa, ovary, and prostate. Its expression is frequently reduced in non-small-cell lung carcinomas and leukemic blasts due to hemizygous gene deletion, supporting its tumor-suppressive role.
• Post-translational modifications include phosphorylation (as indicated by the Phosphoprotein keyword), which may regulate its activity or stability. Alternative splicing generates isoforms with potentially antagonistic functions; for instance, isoform 2 can antagonize transcriptional activation by isoform 1.
• The protein has a calculated molecular weight of approximately 84 kDa, consistent with the 84 kDa indicated by the antibody product.

Experimental Guidance and Technical Tips

• The immunogen for this antibody covers amino acids 561-760 of human DMTF1 (isoform 1). Researchers should verify detection of specific isoforms of interest, as this region may be present in some splice variants.
• For Western blotting, a band around 84 kDa is expected. Use standard SDS-PAGE and transfer protocols. Consider positive controls such as spleen or testis lysates, where DMTF1 expression is relatively high.
• This antibody is recommended for Western blot (WB) and ELISA. For other applications (e.g., IHC, IF), cross-validation in the relevant sample system is advised.
• Due to the tumor-suppressive role of DMTF1, this antibody can be a useful tool in cancer research, particularly in studies of Ras signaling, cell cycle regulation, and p53 pathways.

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