Anti-DMAP1 Rabbit Monoclonal Antibody for WB, ELISA - Q9NPF5

Core Product Specifications and Parameters

Biological Background: DMAP1 Function and Localization

• DNA methyltransferase 1-associated protein 1 (DMAP1, also known as EAF2, SWC4, MEAF2, DNMAP1, DNMTAP1) functions as both a transcriptional repressor and activator.
• It interacts with HDAC2, providing a mechanism for histone deacetylation in heterochromatin following DNA replication at late firing origins.
• DMAP1 is a component of the NuA4 histone acetyltransferase (HAT) complex, which acetylates nucleosomal histones H4 and H2A, regulating transcription, apoptosis, and DNA repair.
• It potentiates DAXX-mediated repression of glucocorticoid receptor-dependent transcription and participates in the nuclear localization of URI1, enhancing its corepressor activity.
• DMAP1 localizes to both the nucleus and cytoplasm.
• Keywords associated with DMAP1 include chromatin regulator, activator, repressor, growth regulation, and isopeptide bond, highlighting its roles in chromatin remodeling and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 368–467 of human DMAP1; consider potential cross-reactivity with isoforms or orthologs in other species when designing experiments.
• For Western blotting, a band at approximately 53 kDa is expected; always include appropriate positive and negative controls and validate in the relevant sample system.
• ELISA may be used for detection of DMAP1 in human samples; pairing with a suitable detection antibody is recommended.
• DMAP1 can be found in both nuclear and cytoplasmic fractions; subcellular fractionation may be considered for context-dependent studies.
• As a monoclonal antibody, batch-to-batch consistency is expected; however, end users should perform independent validation for their specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, bridging the gap from concept to clinic. The DMAP1 Rabbit Monoclonal Antibody supports critical research into chromatin regulation and cancer diagnostics. We provide validated antibody pairs, bulk ancillary reagents, and custom procurement for rare targets to accelerate your assay development.