Anti-CD271 Rabbit Polyclonal Antibody for IHC-P and ELISA - P08138

Core Product Specifications and Parameters

Biological Background: CD271 Function and Localization

• CD271, also known as p75NTR, Gp80-LNGFR, or TNFRSF16, is a tumor necrosis factor receptor superfamily member that functions as a low-affinity receptor for neurotrophins including NGF, BDNF, NTF3, and NTF4.
• Forms a heterodimeric receptor with SORCS2 that binds precursor forms of NGF, BDNF, and NTF3 with high affinity, while showing much lower affinity for mature neurotrophins. Related references: PMID:24908487
• Plays a dual role in neuronal cell fate, capable of mediating both cell survival and apoptosis depending on context and co-receptor interactions.
• Involved in the inactivation of RHOA, contributing to the regulation of actin cytoskeleton dynamics and cell motility. Related references: PMID:26646181
• Regulates insulin-dependent glucose uptake by modulating GLUT4 translocation to the cell surface in adipocytes and skeletal muscle cells, likely through RAB31 activity (By similarity).
• Necessary for circadian oscillation of clock genes such as BMAL1, PER1, PER2, and NR1D1 in the suprachiasmatic nucleus and liver, linking neurotrophin signaling to biological rhythms. Related references: PMID:23785138
• Together with BFAR, negatively regulates NF-kappa-B and JNK-related signaling pathways, providing a mechanism to control inflammatory and stress responses. Related references: PMID:22566094
• Subcellular localization includes the cell membrane, cytoplasm, growth cones, dendritic spines, and perikaryon, reflecting its diverse roles in neuronal signaling and structure.
• Characterized by a signal peptide, four extracellular cysteine-rich repeats, a single transmembrane domain, and a cytoplasmic death domain; undergoes post-translational modifications such as glycosylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 330-427 of human CD271 (intracellular domain), which may provide specific detection of the full-length receptor. For IHC-P, consider using heat-induced antigen retrieval in citrate buffer (pH 6.0) and validate staining in positive control tissues such as human brain, nerves, or melanoma samples.
• For ELISA applications, this polyclonal antibody can be used as a capture or detection reagent. Optimize coating antigen (recombinant CD271 protein or cell lysates) and antibody titration to achieve optimal sensitivity. Cross-reactivity with related neurotrophin receptors should be assessed.
• As a rabbit polyclonal antibody, lot-to-lot consistency should be verified in the intended assay system. Include appropriate negative controls (e.g., tissue from CD271-knockout models or non-expressing cell lines) to confirm specificity.
• Note that p75NTR may undergo cleavage, releasing intracellular domain fragments; for detection of cleaved forms, additional validation with specific antibodies may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, including this rabbit anti-CD271 polyclonal antibody, to support research in neurobiology, apoptosis, and circadian rhythm. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, enabling streamlined assay development from concept to clinical application.