Anti-CD27 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P26842

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 (TNFRSF7) is a single-pass type I membrane protein and member of the tumor necrosis factor receptor superfamily, functioning as a costimulatory immune-checkpoint receptor primarily expressed on T-cells, NK-cells, and B-cells. Related references: PMID:28011863
• Upon binding its ligand CD70/CD27L on B-cells, CD27 activates the TRAF2-PTPN6 axis, which inhibits LCK phosphorylation to drive phenotypic and transcriptional adaptations essential for T-cell memory formation (PubMed:38354704). Related references: PMID:38354704
• Beyond T-cell activation, CD27 engagement on early progenitor cells provides a negative feedback signal to leukocyte differentiation, thereby modulating hematopoiesis (By similarity), and negatively regulates Th2 lymphocyte function in adipose tissue (By similarity).
• The receptor is a glycoprotein and phosphoprotein with three cysteine-rich domains (disulfide bonds) and is localized to the cell membrane.
• It is broadly expressed in most T-lymphocytes and has been associated with apoptosis signaling and disease variants, including lymphoproliferative syndrome 2 (LPFS2).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20-191) of human CD27, suggesting the antibody recognizes the ligand-binding region; consider this when designing competitive assays or epitope mapping studies.
• For Western blotting, the predicted molecular weight is 29 kDa; however, glycosylation may cause a shift to higher apparent molecular weights – validate in your sample system with appropriate controls.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval using heat-induced epitope retrieval (HIER) in citrate buffer (pH 6.0) is recommended; optimize incubation times and antibody dilution for best signal-to-noise.
• This polyclonal antibody reacts with human, mouse, and rat CD27, enabling cross-species studies; however, confirm reactivity in your specific model before critical experiments.
• Multiple applications are reported (WB, IF/ICC, IF-P, IHC-P, ELISA); titrate the antibody for your specific assay format and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio facilitates your IVD development pipeline by providing validated antibody pairs, optimized monoclonal/polyclonal antibodies like this CD27 pAb, and bulk ancillary reagents for immune checkpoint research. Our one-stop platform also supports rare-target raw-material sourcing and technical consulting, ensuring seamless progression from assay concept to clinical application.