Rabbit anti-Human CD27 mAb for FC - P26842

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 is a costimulatory immune-checkpoint receptor expressed on T-cells, NK-cells, and B-cells. It binds to its ligand CD70/CD27L to mediate antigen-specific T-cell activation and expansion, providing immune surveillance of B-cells. Related references: PMID:28011863

• Upon CD70 ligation, CD27 activates the TRAF2-PTPN6 axis, which inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. Related references: PMID:38354704

• CD27 negatively regulates the function of Th2 lymphocytes in adipose tissue.

• It is a member of the tumor necrosis factor receptor superfamily (TNFRSF7), characterized by cysteine-rich repeat domains.

• CD27 is localized at the cell membrane.

• It is predominantly expressed in most T-lymphocytes.

• The protein undergoes post-translational modifications including glycosylation and phosphorylation, and contains disulfide bonds and a signal peptide.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 21-192) of human CD27, suggesting the antibody recognizes the native conformation on live cells, suitable for flow cytometry applications.

• As a ready-to-use conjugate, avoid repeated freeze-thaw cycles and protect from prolonged light exposure to maintain fluorophore stability.

• For flow cytometry, titrate the antibody to determine the optimal concentration in your specific experimental system; use appropriate positive and negative controls (e.g., CD27+ and CD27- cell lines or primary cells) to validate performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers seamless access to high-quality IVD raw materials, including this anti-CD27 monoclonal antibody for immunology and oncology applications. We provide validated antibody pairs, bulk ancillary reagents, and custom sourcing of rare-target raw materials, supporting your projects from concept to clinic.