Anti-CD274 Rabbit Polyclonal Antibody for WB and ELISA - Q9EP73

Core Product Specifications and Parameters

Biological Background: CD274 Function and Localization

• CD274 (PD-L1/B7-H1) is a type I transmembrane protein of the immunoglobulin superfamily, encoded by the Cd274 gene in mouse (aliases: B7h1, Pdcd1l1, Pdcd1lg1, Pdl1).
• Acts as a ligand for the inhibitory receptor PD-1 (PDCD1), playing a critical role in maintaining immune tolerance by modulating T-cell activation and limiting effector responses (PubMed:11238124). Related references: PMID:11238124
• May co-stimulate T-cell subsets that primarily produce interleukin-10 (IL10) through a yet unidentified activating receptor (PubMed:11015443, PubMed:12719480). Related references: PMID:11015443, PMID:12719480
• The PDCD1/PD-L1 pathway is exploited by tumors to attenuate anti-tumor immunity; interaction with PD-1 inhibits cytotoxic T lymphocyte (CTL) effector function, and blockade of this pathway reverses T-cell exhaustion, providing a rationale for cancer immunotherapy (PubMed:12218188, PubMed:27281199). Related references: PMID:12218188, PMID:27281199
• Subcellular localization includes the cell membrane, early endosome membrane, and recycling endosome membrane.
• Highly expressed in the heart, thymus, skeletal muscle, and lung; weakly in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B-cells, macrophages, and numerous lymphoid tumor cell lines.
• Structural features include immunoglobulin-like domains, a disulfide bond, and glycosylation; also possesses a signal peptide, a transmembrane helix, and ubiquitination sites (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 19-239) of mouse CD274, covering the region responsible for PD-1 interaction; this design may favor detection of surface-expressed and soluble forms of the protein in mouse samples.
• For Western blotting, consider using reducing SDS-PAGE conditions; the predicted unmodified molecular weight is ~33 kDa, but glycosylation often results in a higher apparent molecular weight (typically 40–50 kDa). Include appropriate controls to confirm band identity.
• When performing ELISA, titrate the antibody to determine optimal working concentrations; validate performance using recombinant CD274 protein or CD274-expressing cells to establish sensitivity and specificity.
• Although cross-reactivity with human and rat is indicated, verify binding characteristics in your specific experimental context, as epitope accessibility may vary across species and sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, technical services, and consulting—supporting diagnostic developers from concept through clinical application. This anti-mouse CD274/PD-L1 polyclonal antibody is part of our immune checkpoint portfolio, which caters to oncology and immunotherapy research needs. Alongside validated antibodies, we supply matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare target raw materials, helping streamline your procurement strategy with a single, reliable partner.