Anti-CD117 Rabbit Polyclonal Antibody for WB, ELISA - P05532

Core Product Specifications and Parameters

Biological Background: CD117 Function and Localization

• CD117, also known as c-Kit or mast/stem cell growth factor receptor Kit, is a receptor tyrosine kinase encoded by the Kit proto-oncogene (SWISS: P05532). It belongs to the type III receptor tyrosine kinase family featuring extracellular immunoglobulin-like domains and an intracellular kinase domain.
• Upon binding its ligand KITLG/SCF, CD117 dimerizes and autophosphorylates, triggering downstream signaling pathways including PI3K-AKT, RAS-MAPK/ERK, and JAK-STAT. These cascades regulate cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and mast cell development.
• Multiple isoforms exist due to alternative splicing. Isoform 1 and isoform 2 are found in bone marrow and spermatogonia; isoform 3 is enriched in hematopoietic stem cells and detected in later stages of spermatogenesis.
• Subcellularly, CD117 localizes primarily to the cell membrane, but can also be internalized and detected in the cytoplasm upon activation or during receptor turnover.
• Post-translational modifications include glycosylation, disulfide bond formation, ubiquitination, and extensive phosphorylation on tyrosine residues. These modifications modulate receptor activity, stability, and signaling output.
• CD117 is widely expressed in hematopoietic stem cells, mast cells, melanocytes, germ cells, and interstitial cells of Cajal. Mutations or aberrant expression are associated with various pathologies including mastocytosis, gastrointestinal stromal tumors, and leukemias.
• Keywords associated with CD117: Alternative splicing, ATP-binding, Cell membrane, Cytoplasm, Disease variant, Disulfide bond, Glycoprotein, Immunoglobulin domain, Kinase, Magnesium, Membrane, Metal-binding, Nucleotide-binding, Phosphoprotein, Proto-oncogene, Receptor, Reference proteome, Repeat, Signal, Transferase, Transmembrane, Transmembrane helix, Tyrosine-protein kinase, Ubl conjugation.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated using a recombinant immunogen spanning amino acids 25–523 of mouse CD117, corresponding to the extracellular domain. It likely recognizes native and denatured forms of the receptor.
• For Western blotting, the predicted molecular weight of CD117 is approximately 109 kDa. Due to glycosylation, the observed band may appear slightly higher or as a diffuse band. Run adequate protein markers and validate the specific band using appropriate controls.
• In ELISA applications, consider indirect or sandwich formats. The polyclonal nature may provide high sensitivity but confirm specificity by testing on CD117-negative lysates or knockout samples if available.
• The antibody cross-reacts with mouse CD117. For studies in human samples or other species, verify sequence homology and perform pilot experiments to assess cross-reactivity.
• As a polyclonal antibody, lot-to-lot consistency may vary. It is advisable to qualify each new batch in your specific assay system and optimize working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and researchers by offering streamlined access to high-quality IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This CD117 rabbit polyclonal antibody supports investigations into stem cell biology, cancer, and hematopoietic disorders. Our supply network can deliver optimized bulk quantities, matched antibody pairs for immunoassay development, and sourcing of rare-target materials to accelerate your concept-to-clinic journey.