Anti-CD74 Rabbit Polyclonal Antibody for WB, ELISA - P04441-2

Core Product Specifications and Parameters

Biological Background: CD74 Function and Localization

• CD74 is also known as the invariant chain (Ii) or MHC class II-associated invariant chain, and this antibody recognizes the short isoform produced by alternative splicing.
• As an MHC class II-associated invariant chain, CD74 plays a role in the assembly, trafficking, and antigen presentation of MHC class II molecules.
• The protein is primarily expressed in thymus and lymph nodes, highlighting its involvement in immune system tissues.
• The calculated molecular weight of this isoform is approximately 24 kDa, which may appear as a band near this size on Western blots.
• The mouse Cd74 gene encodes this protein, with the synonym Ii reflecting its invariant chain function.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning amino acids 56–215 of mouse CD74 (short isoform). Verify its conformational state for optimal assay performance.
• For Western blot, expect a band around 24 kDa; consider potential post-translational modifications or dimerization that may affect migration.
• For ELISA, validate the antibody with the appropriate antigen format and control for cross-reactivity with other isoforms or species, though only mouse reactivity is listed.
• When probing mouse tissue lysates, include thymus or lymph node samples as positive controls and run appropriate loading controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-CD74 rabbit polyclonal antibody serves researchers and diagnostic developers focusing on antigen presentation, immune profiling, or autoimmune disease markers. For rare target raw-material sourcing or custom antibody development, CamelBio provides end-to-end support from concept to clinic.