PE/Cyanine7 Rabbit anti-Mouse/Rat CD27 mAb - P41272

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27, also known as TNFRSF7, is a member of the tumor necrosis factor receptor superfamily.
• The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion, providing immune surveillance of B-cells (PubMed:11062504). Related references: PMID:11062504
• CD70 ligation activates the TRAF2-PTPN6 axis inhibiting LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (By similarity).
• Activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation, modulating hematopoiesis (PubMed:15723067). Related references: PMID:15723067
• Negatively regulates the function of Th2 lymphocytes in the adipose tissue (PubMed:38386557). Related references: PMID:38386557
• Localized at the cell membrane.
• Expressed in thymus and spleen, but not in non-lymphoid tissues; also detected on myeloid progenitor cells (PubMed:8384562, PubMed:15723067). Related references: PMID:8384562 | PMID:15723067
• Contains a signal peptide, transmembrane helix, disulfide bonds, and glycosylation sites; involved in apoptosis signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 24-182) of mouse CD27, so this antibody is expected to recognize the native receptor on live cells, suitable for flow cytometry.
• Optimize antibody titration and use appropriate isotype controls for FC experiments.
• Cross-reactivity with rat CD27 allows dual-species applications; validate in relevant sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, offering validated antibodies, reagents, and technical support from concept to clinic. This CD27 antibody supports immune-checkpoint research; CamelBio can supply optimized antibody pairs, bulk ancillary reagents, and assist with rare target raw material sourcing for diagnostic development.