Rabbit Anti-Human CD27 Monoclonal Antibody for Flow Cytometry - P26842

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 (TNFRSF7, T14) is a costimulatory immune-checkpoint receptor of the tumor necrosis factor receptor superfamily, expressed on the surface of T-cells, NK-cells, and B-cells.
  Related references: PMID:28011863
• Upon binding its ligand CD70 (CD27L) expressed on B-cells, CD27 drives antigen-specific T-cell activation and expansion, enabling immune surveillance of B-cells.
  Related references: PMID:28011863
• Mechanistically, CD70 ligation recruits TRAF2 and activates the PTPN6 phosphatase, which dephosphorylates LCK, thereby promoting transcriptional and phenotypic adaptations required for T-cell memory.
  Related references: PMID:38354704
• In early hematopoietic progenitor cells, CD27 activation by CD70 provides a negative feedback signal that modulates leukocyte differentiation, fine-tuning hematopoiesis.
• CD27 negatively regulates the function of Th2 lymphocytes in adipose tissue.
• As a type I transmembrane glycoprotein, CD27 is localized to the cell membrane and contains extracellular cysteine-rich domains, a single transmembrane helix, and a phosphorylatable intracellular domain; it is involved in apoptosis.
• Found in most T-lymphocytes, CD27 is a pivotal modulator of adaptive immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 21–192) of human CD27; researchers should verify epitope accessibility and potential receptor shedding in their experimental system.
• This ABflo® 500-conjugated antibody is designed for flow cytometry (FC) on human cells. For optimal resolution, titrate the antibody to establish the staining index and include appropriate isotype controls and Fc receptor blocking reagents.
• Given CD27 expression on diverse lymphocyte subsets, combining this antibody with lineage markers (e.g., CD3, CD19, CD56) can help differentiate T-cell, B-cell, and NK-cell populations in multiplex FC panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD manufacturers and research institutions, delivering high-quality raw materials—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For CD27-targeted immuno-oncology and flow cytometry applications, we facilitate reliable supply of anti-CD27 monoclonal antibodies and rare target raw materials, supporting the entire journey from concept to clinical assay development.