APC Rabbit anti-Human CD27 Monoclonal Antibody for FC - P26842

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 (TNFRSF7/T14) is a costimulatory immune-checkpoint receptor expressed on T-cells, NK-cells, and B-cells, where it binds to and is activated by its ligand CD70/CD27L on B-cells. Related references: PMID:28011863
• The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion, which in turn provides immune surveillance of B-cells. Related references: PMID:28011863
• Upon CD70 ligation, CD27 recruits TRAF2 and activates PTPN6, inhibiting LCK phosphorylation and driving transcriptional adaptations essential for T-cell memory formation. Related references: PMID:38354704
• The protein is localized to the cell membrane and is found in most T-lymphocytes.
• Post-translational modifications include disulfide bond formation, glycosylation, and phosphorylation, as annotated in UniProt.
• CD27 also delivers a negative feedback signal to early hematopoietic progenitor cells, thereby modulating leukocyte differentiation during immune activation.
• The mature protein has a calculated molecular weight of approximately 29 kDa (UniProt: 29137 Da).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 21–192 of human CD27, which covers the extracellular ligand-binding domain. Consequently, the antibody is expected to recognize the native CD27 receptor on live cells, making it suitable for flow cytometry (FC) applications.
• For flow cytometry, consider using appropriate Fc receptor blocking reagents to minimize non-specific binding, especially when analyzing lymphocyte populations rich in Fc receptor-expressing cells.
• Validate the APC conjugate in your specific sample type and instrument setup; fluorophore conjugation may subtly alter antibody affinity or background staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for immunology and immuno-oncology research. This anti-CD27 antibody targets a key immune-checkpoint receptor, supporting diagnostic development in T-cell and NK-cell monitoring. Our global supply network also facilitates rare target raw-material sourcing.