thematics Clia / Elisa

clia / elisa

Enzyme-linked immunosorbent assay (ELISA): The target molecule is quantitatively analyzed by fixing the antibody or antigen on a solid phase carrier, then adding the test sample and enzyme-labeled antibody or antigen, and finally the enzyme catalyzes the substrate to produce a measurable signal (such as color change).

Chemiluminescence Immunoassay (CLIA): By combining antibodies or antigens with chemiluminescent substances, when the target molecules in the sample to be tested bind to the luminescent substances, a light signal is generated through a chemical reaction, thereby quantitatively analyzing the target molecules.

Name Disease Action
C-reactive protein (CRP) Pneumophila Details
Hepatitis C Virus Core (HCV-core) Hepatitis C Virus (hcv) Details
procalcitonin (PCT) Pathogenic Microorganism Infection Details
Anti-Hepatitis B virus (HBsAg) Hepatitis B Details
Anti-Hepatitis B virus (HBeAg) Hepatitis B Details
Hepatitis B virus core antibody (HBcAb) Hepatitis B Details
Immunoglobulin E (IgE) Pathogenic Microorganism Infection Details
Anti-Dengue virus (NS1) Dengue Details
25-hydroxy vitamin D (Anti-Vitamin 25 OH) Details
The thyroid-stimulating hormone (TSH) Thyroid Function Details
NT-proBNP Heart Disease Details
Cystatin C (CysC) Renal Impairment Details
Fatty Acid Binding Protein (H-FABP) Heart Disease Details
Triiodothyronine (T3) Thyroid Conditions Details
thyroxine (T4) Thyroid Conditions Details

Advantages of CLIA (chemiluminescent immunoassay):

  • High sensitivity: The intensity of the chemiluminescent signal is usually stronger than the color change signal produced by the enzyme-catalyzed substrate, so CLIA has higher sensitivity and can detect lower concentrations of target molecules.
  • Low background interference: Chemiluminescent reactions usually have lower background signals, which makes the test results more accurate and reliable.
  • Fast: Chemiluminescent reactions can usually be completed in a shorter time, thereby speeding up the detection.
  • High degree of automation: CLIA technology can usually be combined with automated instruments to achieve high-throughput and high-efficiency detection.
  • Wide application: CLIA technology can be used to detect a variety of biomolecules, including proteins, hormones, viral antigens, etc.

Advantages of ELISA (enzyme-linked immunosorbent assay):

  • Low cost: The reagents and equipment required for ELISA are relatively cheap, suitable for large-scale application and popularization.
  • Simple operation: ELISA technology is relatively simple, easy to learn and operate, and suitable for laboratory and clinical environments.
  • Wide applicability: ELISA can be used to detect a variety of biomolecules, including antigens, antibodies, hormones, cytokines, etc.
  • Quantitative analysis: ELISA can provide quantitative results and is suitable for applications that require accurate measurement of the concentration of target molecules.
  • Good stability: ELISA reagents and reaction conditions are relatively stable, and the results are reproducible.
  • Visual results: ELISA usually displays results through color changes, which is intuitive and easy to interpret.

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