Anti-CMIP Polyclonal Antibody for WB, IHC-P, ELISA - Q8IY22

Core Product Specifications and Parameters

Biological Background: CMIP Function and Localization

• CMIP (C-Maf-inducing protein) exists in at least two isoforms generated by alternative splicing: isoform 1 (full-length) and isoform 2 (truncated, also known as Tc-Mip).
• It functions in T-cell signaling pathways; isoform 2 specifically links T-cell receptor-mediated signals to the activation of the c-Maf transcription factor in T-helper 2 (Th2) cells.
• The protein contains leucine-rich repeats (LRRs), which are commonly involved in protein-protein interactions.
• CMIP localizes to both the nucleus and the cytoplasm, suggesting it may shuttle between compartments to regulate transcriptional and signaling events.
• Tissue expression: isoform 1 is found in peripheral blood mononuclear cells and kidney, with lower levels in brain and liver; its expression is down-regulated upon cell activation. Isoform 2 is expressed in lymphocyte precursors but is silenced during maturation and differentiation in thymus and fetal liver.
• CMIP is classified as a phosphoprotein, and its regulation is likely governed by phosphorylation events during immune signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 640–773 of human CMIP (NP_938204.2), a region that may be conserved across species; cross-reactivity with mouse and rat is reported.
• For WB, consider using whole-cell lysates from T-cell lines or PBMCs; the predicted molecular weight is ~86 kDa.
• IHC-P may require optimized antigen retrieval and primary antibody dilution; testing on lymphoid tissues (e.g., spleen, lymph node) is recommended.
• ELISA protocols should include appropriate controls and titration of the antibody to determine optimal working concentrations.
• Due to isoform-specific expression, confirm band patterns or staining in relevant cell types (e.g., activated vs. resting T cells).
• Store antibody at recommended conditions (ice bag during shipping suggests 4°C short-term; consult product insert for long-term storage).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies the procurement of IVD raw materials by offering a comprehensive range of antibodies, including this CMIP polyclonal antibody, to researchers and diagnostic developers studying T-cell signaling and immune disorders. Our supply capabilities span validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials—all designed to accelerate your project from concept to clinic. Trust CamelBio as your single partner for seamless, reliable access to high-quality immunoreagents.