Anti-Olig2 Rabbit Monoclonal Antibody for WB, ELISA - Q13516

Core Product Specifications and Parameters

Biological Background: Olig2 Function and Localization

• Olig2 is a class B basic helix-loop-helix (bHLH) transcription factor, also designated BHLHB1, bHLHe19, PRKCBP2, or RACK17, that binds DNA to regulate gene expression.
• It is essential for oligodendrocyte and motor neuron specification in the spinal cord and for somatic motor neuron development in the hindbrain.
• Olig2 cooperates with ZNF488 to drive oligodendrocyte differentiation and works alongside OLIG1 to establish the pMN progenitor domain of the embryonic neural tube.
• During cortical development, it controls progenitor fate decisions in gliogenesis and contributes to gliomagenesis; it is classified as a proto-oncogene subject to chromosomal rearrangements.
• The protein acts as a transcriptional repressor of GSX2 by binding and silencing multiple conserved enhancer elements, thereby suppressing olfactory bulb interneuron intermediate progenitor generation.
• It is found in both the nucleus and the cytoplasm, consistent with its DNA-binding role and potential shuttling between compartments.
• Olig2 is predominantly expressed in the brain, notably in oligodendrocytes; it is highly expressed in oligodendrogliomas, with variable levels in astrocytomas and glioblastomas.
• Key post-translational modifications include ubiquitin-like conjugation, and the protein has been identified in proteomic screens.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the internal region (aa 200–300) of human Olig2; the antibody is expected to recognize the full-length protein at ~32 kDa in Western blotting.
• For WB, use lysates from human, mouse, or rat brain tissue or Olig2-expressing cell lines (e.g., oligodendroglioma) and validate with appropriate loading controls.
• In ELISA, consider using the immunogen peptide or recombinant Olig2 protein as a standard and optimize coating conditions to ensure specificity.
• Since the antibody cross-reacts with mouse and rat, it is suitable for comparative and cross-species studies; verify reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-Olig2 monoclonal antibody as part of a comprehensive portfolio for oligodendrocyte differentiation and gliomagenesis research. We support diagnostic manufacturers and laboratories with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom raw-material sourcing. Our one-stop platform accelerates your transition from concept to clinic by providing dependable IVD components and technical expertise.