Anti-Catalase Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04040

Core Product Specifications and Parameters

Biological Background: Catalase Function and Localization

• Catalase (CAT) is a ubiquitous heme-containing antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H₂O₂) into water and oxygen, protecting cells from oxidative damage. Related references: PMID:7882369
• It also functions as a mitogen, promoting the growth of various cell types including T-cells, B-cells, myeloid leukemia cells, melanoma cells, and fibroblasts. Related references: PMID:7882369
• Catalase is primarily localized to the peroxisome matrix, where it neutralizes H₂O₂ generated by peroxisomal oxidases.
• The enzyme contains a heme prosthetic group and binds iron and NADP, classifying it as an oxidoreductase with peroxidase activity.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and stability.
• The predicted molecular weight of human catalase is approximately 60 kDa (59756 Da per UniProt), consistent with the detection of a 60 kDa band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment spanning the N-terminal region (amino acids 1-225) of human catalase. This region is likely surface-exposed and conserved, making it a suitable target for generating antibodies that detect both endogenous and recombinant protein.
• For western blotting (WB), a band at approximately 60 kDa is expected; validate the antibody using appropriate positive control lysates (e.g., human, mouse, or rat tissue/cell lines known to express catalase).
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization conditions, as catalase is localized in peroxisomes. Use tissue sections or cells with high peroxisomal content, such as liver or kidney.
• For ELISA, consider using the antibody as either capture or detection antibody in a sandwich format; pairing with another anti-catalase antibody may improve sensitivity. Always verify performance under your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, providing high-quality antibodies like this anti-Catalase rabbit polyclonal antibody. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing for oxidative stress and peroxisomal research. Whether you need routine supply or custom solutions, CamelBio streamlines your procurement from concept to clinic.