Anti-BOLL Polyclonal Antibody for WB, ELISA - Q8N9W6

Core Product Specifications and Parameters

Biological Background: BOLL Function and Localization

• BOLL (BOULE) is a testis-specific RNA-binding protein that belongs to the family of germ cell-specific RNA-binding proteins.
• It plays a critical role in spermatogenesis, likely by binding to the 3'-UTRs of target mRNAs to regulate their translation.
• The protein localizes to the cytoplasm, where it carries out its post-transcriptional regulatory functions.
• Expression is restricted to the testis, first appearing in spermatocytes and persisting through meiosis; it is absent in early embryos, primordial germ cells, and spermatogonia.
• BOLL is associated with developmental processes and cell differentiation, and multiple isoforms are produced by alternative splicing.
• The predicted molecular weight of human BOLL is approximately 31.3 kDa.

Experimental Guidance and Technical Tips

• For Western blot, consider using tissue-specific positive (testis) and negative (non-testis) controls to confirm specificity, especially when analyzing samples from human, mouse, or rat.
• In ELISA assays, direct coating with the immunogen or recombinant full-length protein may be used as a positive control; cross-species reactivity should be validated in the relevant biological matrix.
• Since the antibody is polyclonal and may recognize multiple epitopes, optimize the dilution series for each application and validate in the target species.
• Alternative splicing may produce variants with different molecular sizes; verify the detected band sizes against known isoforms and predicted masses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-BOLL product, bulk ancillary reagents, and rare target sourcing support. Whether you are developing diagnostic assays for spermatogenesis biomarkers or conducting germ cell research, our integrated supply chain and technical consulting can help bridge the gap from concept to clinic.