α-Synuclein Rabbit pAb - P37840

Core Product Specifications and Parameters

Biological Background: Alpha-synuclein Function and Localization

• Alpha-synuclein is a neuronal protein highly expressed in presynaptic terminals of the central nervous system, principally in the brain.
• It regulates synaptic vesicle trafficking and neurotransmitter release, playing a key role in synaptic activity.
  Related references: PMID:20798282, PMID:26442590, PMID:28288128
• As a monomer, alpha-synuclein enhances synaptic vesicle exocytosis by promoting vesicle priming, fusion, and dilation of exocytotic fusion pores; it acts by increasing local Ca²⁺ release from microdomains.
  Related references: PMID:28288128, PMID:30404828
• In its membrane-bound multimeric state, alpha-synuclein functions as a molecular chaperone, assisting in the folding of SNARE proteins at the presynaptic membrane in cooperation with cysteine string protein-alpha (DNAJC5); this chaperone activity is important to sustain SNARE-complex assembly during aging.
  Related references: PMID:20798282
• Alpha-synuclein modulates dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby regulating its activity.
  Related references: PMID:26442590
• Subcellularly, it localizes to the cytoplasm, membrane, nucleus, and synapse; it is also secreted and found in cell projections including axons.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination; alpha-synuclein is also associated with amyloid formation in Alzheimer's disease.

Experimental Guidance and Technical Tips

• The immunogen spans the central region (aa 59–140) of alpha-synuclein, avoiding the highly charged N‑terminus and aggregation‑prone C‑terminus; consider using complementary detection reagents targeting other epitopes if needed.
• For Western blot (WB), the predicted molecular weight is ~14 kDa; validate detection in relevant brain tissue or neuronal cell lysates.
• In immunofluorescence applications (IF/ICC and IF‑P), optimize fixation and permeabilization protocols to detect alpha‑synuclein in presynaptic terminals; cross‑reactivity with mouse and rat supports use in rodent model studies.
• ELISA applications are listed; appropriate antigen coating and detection conditions should be established for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by providing one‑stop access to high‑quality raw materials and technical services. For neurodegenerative disease research and diagnostic development targeting alpha‑synuclein, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Whether you require rare target sourcing or customized solutions, CamelBio supports your journey from concept to clinic.