Lumican (LUM) Rabbit pAb for WB, IF/ICC, ELISA - P51884

Core Product Specifications and Parameters

Biological Background: Lumican Function and Localization

• Lumican is a keratan sulfate proteoglycan also known as KSPG lumican, belonging to the small leucine-rich repeat proteoglycan (SLRP) family.
• It is encoded by the LUM gene (aliases LDC, SLRR2D) in humans.
• The protein is secreted and predominantly localized to the extracellular space and extracellular matrix.
• Tissue specificity includes expression in the cornea and various other tissues.
• Key structural features include leucine-rich repeats, disulfide bonds, and sites for sulfation and phosphorylation, classifying it as a glycoprotein and proteoglycan.
• The mature protein undergoes N‑terminal pyroglutamate formation and has a calculated molecular weight of approximately 38.4 kDa (pre‑post‑translational modifications).
• It contributes to extracellular matrix organization and maintenance, particularly in collagen‑rich tissues.
• Its presence in the cornea suggests roles in maintaining corneal transparency and matrix architecture.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 19–338 of human lumican (NP_002336.1), representing nearly the full‑length mature protein. This design may favor recognition of native lumican in biological samples.
• For Western blotting (WB), the observed band is expected around 38 kDa; however, due to glycosylation and proteoglycan modifications, the apparent molecular weight may differ in certain tissue or cell lysates.
• The antibody is reported to react with human, mouse, and rat lumican; sequence homology should be verified if used with other species.
• For immunofluorescence/immunocytochemistry (IF/ICC), extracellular matrix staining patterns are anticipated. Optimization of fixation and permeabilization may be required to preserve epitopes.
• In ELISA applications, the recombinant immunogen can serve as a positive control; titration of both capture and detection antibodies is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by providing verified antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for lumican and other extracellular matrix targets. Whether you require high‑quality raw materials for assay development or seek rare‑target sourcing solutions, our integrated supply chain bridges your project from concept to clinic. Contact us to explore how we can accelerate your extracellular matrix or ophthalmology‑related IVD programs.