Anti-Tau Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Tau (Microtubule-associated protein tau) promotes microtubule assembly and stability and is involved in the establishment and maintenance of neuronal polarity. Related references: PMID:21985311
• Tau acts as a linker protein, with its C-terminus binding axonal microtubules and its N-terminus binding neural plasma membrane components. Related references: PMID:21985311 PMID:32961270
• Axonal polarity is determined by TAU/MAPT localization near the centrosome; short tau isoforms provide cytoskeletal plasticity, while longer isoforms preferentially stabilize microtubules.
• Expressed primarily in neurons; a peripheral nervous system-specific isoform (PNS-tau) is expressed in the PNS, while other isoforms are central nervous system-specific.
• Subcellular localization includes cytoplasm, cytosol, cell membrane, cytoskeleton, axons, dendrites, and secretion.
• Post-translational modifications include phosphorylation, acetylation, glycation, methylation, O-glycosylation, and ubiquitination; mutations and hyperphosphorylation are linked to Alzheimer disease, Parkinsonism, and other tauopathies.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 658–758 of human Tau, a C-terminal region; this design may detect multiple isoforms due to alternative splicing.
• In Western blot, expect a primary band near 79 kDa, though post-translational modifications (e.g., phosphorylation) and splice variants could yield additional bands.
• For immunohistochemistry and immunofluorescence, validate in appropriate neuronal tissue or cell models; cross-reactivity with mouse and rat supports use in rodent studies.
• Consider ELISA for quantitative analysis, but note that epitope accessibility may vary with sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. Our portfolio supports neurodegeneration research, including tau biology and Alzheimer’s disease biomarkers. We also assist in sourcing rare raw materials, streamlining your transition from concept to clinic.