Anti-Syntaxin 3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q13277

Core Product Specifications and Parameters

Biological Background: Syntaxin 3 Function and Localization

• Syntaxin-3 (STX3) belongs to the syntaxin family of SNARE proteins and plays a role in vesicle trafficking and membrane fusion. Alternative splicing produces multiple isoforms with distinct tissue distributions.
• It is potentially involved in docking of synaptic vesicles at presynaptic active zones, although it is not expressed in brain and skeletal muscle, suggesting a role in non-neuronal exocytosis.
• Syntaxin-3 functions as an apical receptor critical for membrane fusion of apical vesicles in polarized epithelial cells, contributing to cell polarity and sorting.
• Essential for survival of retinal photoreceptors; mutations in STX3 are associated with retinal degeneration and microvillus inclusion disease.
• Additionally, Syntaxin-3 acts as a regulator of gene expression, indicating a nuclear role beyond membrane trafficking.
• Subcellular localization includes the apical cell membrane and the nucleus, consistent with its transmembrane domain and nuclear translocation.
• Tissue-specific expression: Isoform 1 is found in small intestine, kidney, pancreas, placenta, and retina; isoform 2 is retina-specific; isoform 3 is ubiquitously expressed.
• The protein contains a coiled-coil domain and a transmembrane helix; it is annotated with keywords: Cell membrane, Disease variant, Neurotransmitter transport, Nucleus, and Transport.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of Syntaxin-3 is approximately 33 kDa. Use appropriate protein loading and verify the band with positive control lysates (e.g., HeLa, kidney, or retinal tissue).
• In immunohistochemistry (paraffin sections), Syntaxin-3 localizes to apical cell membranes and nuclei. Antigen retrieval with citrate buffer (pH 6.0) may enhance detection; validate with known positive and negative tissues.
• For ELISA applications, this antibody can be used as a capture or detection reagent; pair with a matched antibody specific to a distinct epitope for sandwich ELISA. Titrate concentrations to optimize signal-to-noise.
• Given the immunogen (peptide from amino acids 100–200), the epitope likely resides in a region conserved across human, mouse, and rat; confirm cross-reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive IVD raw-material supply chain, delivering high-quality monoclonal antibodies like this Syntaxin 3 Rabbit mAb to diagnostic developers and researchers. Our platform provides flexible bulk purchasing, validated antibody pairs, and ancillary reagents, streamlining your assay development. With expertise in rare target sourcing, we support your projects from early-stage research to commercialization.