Anti-STX1A Polyclonal Antibody for WB, IF/ICC, ELISA - Q16623

Core Product Specifications and Parameters

Biological Background: Syntaxin-1A Function and Localization

• Syntaxin-1A (STX1A), also known as HPC-1, is a Q-SNARE protein and a key member of the syntaxin family.
• Essential for calcium-dependent exocytosis of neurotransmitters and hormones; forms the SNARE complex with SNAP25 and VAMP2 to drive synaptic vesicle fusion with the plasma membrane (PubMed:26635000). Related references: PMID:26635000
• Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm, highlighting its role beyond neuronal tissues (PubMed:23091057). Related references: PMID:23091057
• Involved in the regulated secretion of insulin and glucagon-like peptide 1 (GLP-1), linking it to metabolic processes.
• Subcellular distribution includes synaptic vesicle membranes, the presynaptic plasma membrane, and secretory vesicles; it is also secreted under certain conditions.
• Tissue expression is widespread: highly expressed in embryonic spinal cord and ganglia, adult cerebellum and cerebral cortex, and additionally in heart, liver, fat, skeletal muscle, kidney, and brain.
• Key post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitination; structural features include a transmembrane domain and a coiled-coil region. Aliases: STX1, P35-1, SYN1A.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-288 of human STX1A, covering a large portion of the protein; thus, the polyclonal antibody may recognize multiple epitopes, potentially enhancing sensitivity in applications such as Western blotting.
• For Western blot (WB), a band at approximately 33 kDa is expected; use whole-cell or tissue lysates from human or rat samples, and include appropriate loading controls and molecular weight markers.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimal fixation and permeabilization conditions should be determined empirically, as STX1A localizes to the plasma membrane and intracellular vesicles.
• For ELISA, the recombinant immunogen can be used as a positive control to establish assay parameters.
• Given reported cross-reactivity with rat, validation in rat-derived tissues or cell lines is feasible; however, cross-reactivity with other species should be independently tested if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality reagents like this anti-STX1A polyclonal antibody. Beyond single antibodies, we supply validated antibody pairs, optimized monoclonals, and bulk ancillary reagents to support your neuroscience and exocytosis research programs. Our technical team also assists in sourcing rare-target raw materials, ensuring continuity from concept to clinic.