Anti-PKC zeta Polyclonal Antibody for WB, IF/ICC, ELISA - Q05513

Core Product Specifications and Parameters

Biological Background: PKC zeta Function and Localization

• PKC zeta (nPKC-zeta) is an atypical protein kinase C, lacking calcium and diacylglycerol sensitivity, and functions as a serine/threonine-protein kinase in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response, and maintenance of long-term potentiation (LTP).
• Downstream of PI3K, PKC zeta activates the MEK1-ERK2 cascade independently of RAF1 in macrophages and following mitogenic stimuli. It also contributes to insulin-dependent AKT3 activation and GLUT4 translocation in adipocytes, acting partly as an adapter.
• PKC zeta mediates NF-kappa-B activation via interactions with SQSTM1/p62, RIPK1, and TRAF6, leading to IKBKB phosphorylation and NF-kappa-B inhibitor degradation. It further phosphorylates RELA at Ser-311 to counteract SETD6-mediated repression.
• In cell polarity, PKC zeta forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to establish polarity with microtubule motors. It stimulates neuronal differentiation with FEZ1 and is required for late-phase LTP in hippocampal CA1 synapses, crucial for long-term memory.
• PKC zeta phosphorylates VAMP2 in vitro and activates LRRK1, which then phosphorylates RAB proteins involved in intracellular trafficking. Related references: PMID:17313651 PMID:36040231
• PKC zeta localizes to the cytoplasm, endosomes, cell junctions, and membrane. It is predominantly expressed in the brain, with lower levels in lung, kidney, and testis. It contains a zinc-finger domain and ATP-binding site, and is subject to alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 476–547), which may provide good epitope accessibility for native and denatured protein detection.
• For Western blotting, the predicted molecular weight is ~68 kDa; always include positive and negative controls to confirm specificity.
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions, as PKC zeta can localize to multiple compartments (cytoplasm, membrane, endosomes, junctions).
• ELISA can be used for quantitative detection of PKC zeta in cell lysates or purified protein; validate with known standards.
• Cross-reactivity with mouse and rat is expected; confirm reactivity in your specific sample system before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and research labs with convenient access to IVD raw materials, technical services, and consulting from concept to clinic. This anti-PKC zeta antibody supports investigations into cell signaling, cancer biology, and inflammatory pathways. Beyond single reagents, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development workflow.