Anti-BSN Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UPA5

Core Product Specifications and Parameters

Biological Background: BSN (Bassoon) Function and Localization

• BSN encodes protein bassoon (also known as zinc finger protein 231), a large scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) where neurotransmitter is released (PubMed:12812759).
  Related references: PMID:12812759
• After synthesis, bassoon participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (PubMed:19380881).
  Related references: PMID:19380881
• At the presynaptic active zone, bassoon regulates the spatial organization of synaptic vesicle clusters, the protein complexes that execute membrane fusion, and compensatory endocytosis.
• Bassoon also plays roles in regulating presynaptic protein ubiquitination by interacting with SIAH1 and in regulating presynaptic autophagy by associating with ATG5.
• Bassoon mediates synapse-to-nucleus communication by associating with the transcriptional corepressor CTBP1, reducing the size of its pool available for nuclear import and thereby reconfiguring gene expression.
• The protein inhibits the activity of the proportion of DAO enzyme that localizes to the presynaptic active zone, which may modulate synaptic transmission.
• BSN is exclusively expressed in brain tissue, consistent with its critical synaptic functions.

Experimental Guidance and Technical Tips

• Due to the large molecular weight of bassoon (approximately 416 kDa), consider using low-percentage SDS-PAGE gels (e.g., 6% or gradient gels) and extended transfer times for western blotting.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization protocols to preserve synaptic structures; bassoon localizes to presynaptic active zones, so co-staining with synaptic markers is recommended.
• The immunogen corresponds to amino acids 830-950 of human BSN; verify antibody specificity in relevant knockout or knockdown models.
• This antibody shows cross-reactivity with mouse BSN, making it suitable for translational studies in mouse brain tissue.
• For ELISA applications, validate the antibody pair compatibility and assay conditions with purified recombinant protein or native tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, from concept to clinic. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target materials such as this anti-BSN pAb for neuroscience research. Trust CamelBio to streamline your procurement and accelerate your diagnostic development.