Anti-BRCA1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P38398

Core Product Specifications and Parameters

Biological Background: BRCA1 Function and Localization

• BRCA1 is a breast cancer type 1 susceptibility protein that functions as a RING finger protein and E3 ubiquitin-protein ligase, specifically mediating 'Lys-6'-linked polyubiquitin chain formation, which is crucial for DNA damage repair and genomic stability. Related references: PMID:10500182, PMID:12887909, PMID:12890688

• The BRCA1-BARD1 heterodimer orchestrates DNA damage repair, ubiquitination, and transcriptional regulation, ensuring genomic integrity. Related references: PMID:12890688, PMID:14976165, PMID:20351172

• BRCA1 is required for ionizing radiation-induced cell cycle arrests in both S-phase and G2 phase, contributing to proper checkpoint control. Related references: PMID:10724175, PMID:11836499, PMID:12183412

• It regulates centrosomal microtubule nucleation, impacting chromosome segregation and mitotic fidelity. Related references: PMID:18056443

• BRCA1 facilitates homologous recombination repair by interacting with PALB2, which promotes BRCA2-RAD51 loading at DNA breaks. Related references: PMID:19369211

• Additionally, BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and acts as a transcriptional activator to modulate gene expression. Related references: PMID:16326698, PMID:20160719

• The protein undergoes phosphorylation and acetylation and contains a RING-type zinc finger domain essential for its E3 ligase activity. BRCA1 localizes predominantly to the nucleus and chromosomes, with some cytoplasmic presence. Isoform 1 and 3 are widely expressed, while isoform 3 is often reduced or absent in breast and ovarian cancer cell lines.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 901-1000; this central region may recognize full-length BRCA1 and potentially some isoforms.
• For Western blot, consider using whole-cell lysates from cell lines with known BRCA1 expression (e.g., HeLa, MCF-7) due to the large ~208 kDa protein size; ensure proper gel electrophoresis and transfer conditions.
• In immunohistochemistry (IHC-P), antigen retrieval may be required; validate the antibody in frozen or paraffin-embedded tissues according to standard protocols.
• For immunofluorescence/ICC, fixation and permeabilization conditions should be optimized for nuclear and cytoplasmic detection.
• ELISA can be used for detecting recombinant BRCA1 fragments or screening antibodies; consider paired antibody strategies for sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for assays targeting BRCA1 in oncology and genetic testing. Whether you need bulk quantities or specialized sourcing for rare targets, CamelBio supports your diagnostic development from concept to clinic.