SRBD1 Rabbit pAb - Q8N5C6

Core Product Specifications and Parameters

Biological Background: SRBD1 Function and Localization

• SRBD1 (S1 RNA-binding domain-containing protein 1) is a DNA- and histone-binding protein that localizes to the central axes of mitotic chromosomes, playing a critical role in chromosome architecture during cell division. Related references: PMID:39955279, PMID:40106440
• It promotes chromosome segregation by facilitating the recruitment of topoisomerase II alpha (TOP2A) to mitotic chromosomes, thereby preventing anaphase failure. Related references: PMID:39955279
• During prophase, SRBD1 safeguards the decatenation process to avoid the formation of difficult-to-resolve DNA structures, preventing chromosome missegregation. Related references: PMID:40106440
• SRBD1 also contributes to the regulation of nucleus pulposus cell senescence, indicating a role in cellular aging and intervertebral disc biology. Related references: PMID:38169523
• Subcellularly, SRBD1 is found in the nucleus, nucleolus, cytoplasm, and on chromosomes, consistent with its multifaceted roles in chromatin dynamics and cell cycle regulation.
• The protein contains an S1 RNA-binding domain and a coiled-coil region, and is subject to post-translational modifications including phosphorylation and ubiquitin-like conjugation (isopeptide bond).
• Alternative splicing of SRBD1 generates multiple isoforms, which may fine-tune its functions in different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen used (amino acids 330–600) covers a region of SRBD1 that may include functional domains such as the coiled-coil; therefore, the antibody might recognize both full-length and certain isoform-specific bands. Validate in your specific sample system.
• For Western blot (WB), consider using whole cell lysates from human, mouse, or rat cell lines, as cross-reactivity with these species is reported based on sequence homology. Optimize loading amounts and detection conditions.
• For ELISA, the polyclonal nature of this antibody may provide robust signal amplification; however, perform a standard checkerboard titration to determine the optimal coating and detection antibody concentrations.
• SRBD1 expression may vary with cell cycle stage or senescence; thus, synchronizing cells or using senescence models could enhance detection reliability. Include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. For chromosome biology and cell cycle targets like SRBD1, we offer validated polyclonal antibodies, custom antibody development, and ancillary reagents to streamline your assay development. As your dedicated sourcing partner, we bridge the gap from target discovery to clinical application, ensuring reliable supply for your workflow needs.