MonoMethyl-Histone H3-K79 Rabbit Polyclonal Antibody (Q16695 / P68431) for WB, ELISA, ChIP

Core Product Specifications and Parameters

Biological Background: MonoMethyl-Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This compaction restricts DNA accessibility, thereby regulating fundamental nuclear processes including transcription, DNA repair, replication, and chromosomal stability.
• As a central player in the "histone code," H3 undergoes numerous site-specific post-translational modifications (PTMs) — such as methylation, acetylation, phosphorylation, and citrullination — that fine-tune chromatin structure and gene expression. Monomethylation at lysine 79 (K79me1) represents one such regulatory mark implicated in transcriptional control and epigenetic memory.
• Histone H3 localizes primarily to the nucleus and is an integral component of chromosomes. Multiple H3 variants exist in humans: canonical replication-dependent H3.1 (encoded by several HIST1H3 genes and P68431) and the testis-specific H3.1t (Q16695), which is selectively expressed in testicular cells.
• The precise function of K79 monomethylation is context-dependent; it has been associated with both active and repressed chromatin environments, and its aberrant regulation is linked to developmental disorders and cancers.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a synthetic monomethylated peptide corresponding to residues around K79 of human histone H3 (NP_003520.1), ensuring specificity for the K79me1 mark.
• Western blotting (WB): For detection, consider using nuclear protein extracts or acid-extracted histones. Given the predicted molecular weight of approximately 15 kDa, standard SDS-PAGE and transfer protocols are suitable. Test a range of antibody dilutions to optimize signal-to-noise.
• ELISA: Use synthetic monomethylated peptides and appropriate blocking buffers to minimize non-specific binding. Include unmodified and other modified peptides as controls to confirm specificity.
• Chromatin immunoprecipitation (ChIP): Cross-linking conditions and sonication should be carefully optimized to preserve the methyl mark and achieve appropriate chromatin fragmentation. Validate antibody performance in the relevant species (human, mouse, rat) and cellular context.
• Cross-reactivity: The antibody is predicted to react with a wide range of species due to high sequence conservation of the H3K79 region. Validate specificity in your experimental system using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This MonoMethyl-Histone H3-K79 Rabbit pAb is a methylation-specific research tool for epigenetics studies; CamelBio provides integrated procurement, technical consultation, and custom IVD raw-material supply solutions from concept to clinic. Contact us to explore our comprehensive sourcing services for your next development project.