HNRNPR Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O43390

Core Product Specifications and Parameters

Biological Background: HNRNPR Function and Localization

• HNRNPR, also known as hnRNP R, is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family.
• It is a core component of ribonucleosomes, which are complexes of at least 20 different hnRNPs that assemble on nascent transcripts.
• These ribonucleosome complexes play a critical role in precursor mRNA processing within the nucleus, including splicing and transport.
• HNRNPR contains RNA recognition motifs and is classified as an RNA-binding protein involved in mRNA splicing and metabolism.
• Subcellular localization is primarily nuclear (nucleoplasm) but HNRNPR is also detected in the cytoplasm and associated with microsomal/endoplasmic reticulum fractions.
• Post-translational modifications include acetylation and ubiquitin-like conjugation, and multiple isoforms arise from alternative splicing.
• Genetic variants in HNRNPR have been associated with intellectual disability, underscoring its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide from the C-terminal region (aa 550-633) of human HNRNPR. This sequence is conserved in mouse and rat, consistent with the predicted multi-species reactivity.
• For western blotting (WB), expect a specific band at approximately 71 kDa. Optimize sample preparation and antibody dilution using a positive control lysate.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate signal specificity in tissues or cell lines known to express HNRNPR, such as neuronal cells.
• For ELISA applications, this polyclonal antibody can serve as a capture or detection reagent; consider pairing with a matched monoclonal antibody for sandwich assay development.
• Always include appropriate positive and negative controls to confirm performance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, connecting diagnostic manufacturers and research labs with reliable antibodies and related reagents for mRNA processing research. Whether you need validated anti-HNRNPR antibodies, immune assay development support, or bulk ancillary materials, our integrated supply chain and technical expertise help accelerate your project from concept to clinic. Explore our comprehensive catalog for your HNRNPR-targeted study.