EED Rabbit Polyclonal Antibody for WB, IF, IP, ELISA - O75530

Core Product Specifications and Parameters

Biological Background: EED Function and Localization

• Polycomb protein EED (Embryonic ectoderm development protein), also designated HEED, COGIS, and WAIT-1, is a core component of the polycomb repressive complex 2 (PRC2). Alternative splicing and alternative initiation yield multiple isoforms.
• As part of the PRC2/EED-EZH2 complex, EED methylates histone H3 at lysine-9 and lysine-27, leading to transcriptional silencing of target genes such as HOXC8, HOXA9, MYT1, and CDKN2A.
• EED recognizes trimethylated histone H1 at lysine-26 (H1K26me3), which inhibits PRC2 methyltransferase activity on nucleosomal H3K27, while recognition of H3K27me3 propagates the repressive mark.
• The PRC2/EED-EZH2 complex may also recruit DNA methyltransferases, establishing a functional link between histone modification and DNA methylation for stable gene repression.
• EED is localized to the nucleus and associates with chromosomes, consistent with its role in chromatin regulation.
• It is subject to multiple post-translational modifications, including acetylation, phosphorylation, and methylation, which may modulate its activity and interactions.
• EED is widely expressed across human tissues, with notably elevated levels observed in breast and colon cancer, supporting its involvement in oncogenesis.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 400 amino acids of human EED; this region may detect full-length protein and certain isoforms. Validate detection of any specific splice variants in your experimental system.
• For Western blotting (WB), the predicted molecular weight is approximately 50 kDa. Confirm band size with appropriate positive and negative controls.
• Cross-reactivity is reported for human, mouse, and rat. Verify reactivity with other species if needed by sequence alignment of the immunogen.
• In immunofluorescence/ICC, perform standard fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100). Nuclear staining is expected.
• For immunoprecipitation (IP), use Protein A or G beads and optimize antibody-to-lysate ratio. Pre-clear lysates to reduce background.
• ELISA suitability is indicated; titrate antibody and use recombinant EED protein or peptide as coating antigen.
• Given EED overexpression in certain cancers, include relevant cancer cell lines (e.g., breast, colon) as positive controls.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw materials such as this anti-EED antibody for epigenetic and cancer research. Whether you need a single reagent or a complete custom solution, CamelBio bridges your sourcing needs efficiently.