Anti-YARS Rabbit Polyclonal Antibody for WB, IP, ELISA - P54577

Core Product Specifications and Parameters

Biological Background: YARS Function and Localization

• Encodes cytoplasmic tyrosine--tRNA ligase (tyrosyl-tRNA synthetase, TyrRS), a member of the class-I aminoacyl-tRNA synthetase family essential for protein biosynthesis.
• Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). Also acts as a positive regulator of poly-ADP-ribosylation in the nucleus, independently of its tRNA ligase activity. Activity is switched upon resveratrol-binding: resveratrol strongly inhibits the tyrosine--tRNA ligase activity and promotes relocalization to the nucleus, where YARS1 specifically stimulates the poly-ADP-ribosyltransferase activity of PARP1. Related references: PMID:25533949
• Localizes predominantly in the cytoplasm but undergoes stimulus-dependent translocation to the nucleus.
• Contains ATP-binding and tRNA-binding domains characteristic of aminoacyl-tRNA synthetases; post-translational modifications include acetylation and phosphorylation.
• Genetic variants in YARS1 are associated with Charcot-Marie-Tooth disease type DIC and other neuropathies, highlighting its role in neuronal maintenance.
• Key biological processes: protein translation, tRNA aminoacylation, poly-ADP-ribosylation, and cellular stress responses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (a.a. 1–300) of human YARS, enabling detection of full-length protein as well as potential N-terminal fragments or isoforms.
• For Western blot (WB), consider using whole-cell lysates from human, mouse, or rat cell lines; validate antibody dilution and exposure conditions in your specific sample system.
• In immunoprecipitation (IP), verify the antibody’s efficiency in pulling down endogenous YARS from your chosen cell type, as binding may be influenced by post-translational modifications or binding partners.
• For ELISA, use recombinant YARS protein as a positive control; perform appropriate blocking and washing steps to minimize non-specific binding.
• Because cross-reactivity with mouse and rat has been demonstrated, this antibody is suitable for comparative studies across these species; however, always confirm specificity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to IVD raw materials, technical services, and consulting—from concept to clinic. For studies on aminoacyl-tRNA synthetases, protein synthesis, or neurodegeneration, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare-target raw materials. Let CamelBio be your bridge to reliable, high-quality research supplies.