Anti-TRMT61B Rabbit Polyclonal Antibody for WB, ELISA - Q9BVS5

Core Product Specifications and Parameters

Biological Background: TRMT61B Function and Localization

• TRMT61B is a mitochondrial methyltransferase that catalyzes the formation of N1-methyladenosine (m1A) at position 58 of various mitochondrial tRNAs, including tRNA(Leu), tRNA(Lys), and tRNA(Ser). Related references: PMID:23097428

• It also methylates adenosine at position 947 of mitochondrial 16S ribosomal RNA, a modification likely important for mitoribosomal structure and function. Related references: PMID:27631568

• Additionally, TRMT61B acts as an mRNA N1-methyltransferase, methylating adenosine residues in MT-ND5 mRNA to interfere with mitochondrial translation. Related references: PMID:29107537

• The protein is localized to the mitochondrial matrix, consistent with its role in processing mitochondrial transcripts.

• As a member of the methyltransferase superfamily, TRMT61B utilizes S-adenosyl-L-methionine (SAM) as a methyl donor.

• Alternative names include mRNA methyladenosine-N(1)-methyltransferase, reflecting its activity on both tRNA and mRNA substrates.

• The gene TRMT61B is associated with epitranscriptomic regulation in mitochondria, influencing gene expression at the RNA level.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–250 of human TRMT61B; the polyclonal antibody may recognize multiple epitopes within this N-terminal region.
• For Western blotting, the predicted molecular weight is approximately 53 kDa; consider using appropriate positive controls such as human cell lines with high mitochondrial content.
• Since cross-reactivity is reported only for human samples, validate the antibody in your specific human-derived sample system.
• For ELISA, standard titrations and blocking steps should be optimized; the antibody is rabbit polyclonal, so appropriate secondary detection reagents are required.
• Given the mitochondrial localization, subcellular fractionation or mitochondrial enrichment may enhance detection sensitivity in Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated antibodies for epitranscriptomics and mitochondrial research. Our supply network covers polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents, ensuring you receive reliable materials for applications such as WB and ELISA. For rare targets like TRMT61B, CamelBio offers dedicated sourcing support to help bridge your project needs with global capabilities.