Anti-TRMT6 Polyclonal Antibody for WB, ELISA - Q9UJA5

Core Product Specifications and Parameters

Biological Background: TRMT6 Function and Localization

• TRMT6 is the substrate-binding subunit of the tRNA (adenine-N(1)-)-methyltransferase complex, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Related references: PMID:16043508
• Together with the catalytic subunit TRMT61A, TRMT6 participates in an mRNA N1-methyltransferase complex that methylates adenosine at the N1 position in a small subset of mRNAs, recognizing tRNA-like T-loop structures. Related references: PMID:29072297, PMID:29107537
• TRMT6 is primarily localized in the nucleus, consistent with its role in RNA modification.
• The protein is expressed in multiple tissues, including brain, liver, testis, and ovary.
• TRMT6 is classified as a phosphoprotein, indicating potential post-translational regulation.
• Alternative splicing of TRMT6 produces multiple isoforms, expanding functional diversity.
• It is involved in tRNA processing and is part of the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-200) of human TRMT6. Consider validating antibody specificity using knockdown or knockout controls in relevant cell lines.
• For Western blot, a band near 56 kDa is expected. Optimize sample preparation and loading to account for possible post-translational modifications or isoform variations.
• In ELISA, ensure the coating antigen includes the immunogen region to confirm reactivity. Cross-reactivity with other species is not reported; limit testing to human samples.
• Polyclonal antibodies may exhibit lot-to-lot variability; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a dedicated IVD raw-material partner, CamelBio offers this TRMT6 polyclonal antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your methylation or tRNA biology research and diagnostic development. Let us bridge your sourcing needs with reliable supply and technical expertise.