Anti-TRMT2A/HTF9C Polyclonal Antibody for WB, IHC-P, ELISA - Q8IZ69

Core Product Specifications and Parameters

Biological Background: TRMT2A Function and Localization

• TRMT2A (tRNA (uracil-5-)-methyltransferase homolog A), also known as HTF9C, is a member of the RNA methyltransferase family.
• It catalyzes the formation of 5-methyl-uridine (m5U) in cytoplasmic tRNAs, primarily at position 54 (m5U54), and in a subset of mRNAs, using S-adenosyl-L-methionine as the methyl donor. Related references: PMID:31361898 | PMID:33799331 | PMID:34556860 | PMID:34123281
• The protein contains a coiled-coil domain and undergoes alternative splicing and phosphorylation, contributing to functional diversity.
• It is predominantly localized in the cytoplasm/cytosol, consistent with its role in tRNA and mRNA processing.
• TRMT2A is classified under multiple functional keywords: methyltransferase, transferase, tRNA processing, mRNA processing, and RNA-binding.
• A 3D structure has been resolved, and its molecular weight is approximately 68.7 kDa, matching the observed 69 kDa band in experimental settings.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from the C-terminal region (aa 500–end) of human TRMT2A. In western blot (WB), expect detection of a ~69 kDa band corresponding to full-length TRMT2A.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 8.0) based on tissue type and fixation.
• In ELISA, the antibody can serve as a detection or capture reagent. Validate against recombinant TRMT2A or endogenous lysates from human, mouse, or rat samples.
• Cross-reactivity with mouse and rat has been confirmed; however, due to sequence homology, validate specificity in each species-specific application.
• Since this is a polyclonal antibody, lot-to-lot consistency should be verified when transitioning between production batches.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing for diagnostic manufacturers and research teams, offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For TRMT2A/HTF9C research in RNA modification and epitranscriptomics, we provide consistent supply of this antibody and can assist with rare target sourcing. Partner with us to bridge your project from concept to clinic with reliable materials and technical support.