Anti-TRMT1 Polyclonal Antibody for WB, ELISA - Q9NXH9

Core Product Specifications and Parameters

Biological Background: TRMT1 Function and Localization

• TRMT1 (tRNA methyltransferase 1) is the enzyme responsible for N2,N2-dimethylguanosine (m2,2G26) formation at position 26 of most nuclear- and mitochondrial-encoded tRNAs, a modification critical for tRNA stability and function (PubMed:10982862, PubMed:28784718, PubMed:37204604). Related references: PMID:10982862 PMID:28784718 PMID:37204604
• This tRNA guanine(26)-dimethylation is required for cellular redox homeostasis, proper proliferation, and survival under oxidative stress, linking TRMT1 to central metabolic regulation.
• The enzyme localizes to the nucleus, mitochondria, and cytoplasm, consistent with its dual role in modifying both nuclear- and mitochondrial-encoded tRNAs.
• Alternative splicing of the TRMT1 gene yields multiple protein isoforms, potentially contributing to functional diversity.
• TRMT1 contains a zinc-finger domain indicating metal-binding capacity, and is known to be regulated by phosphorylation.
• Mutations in TRMT1 are associated with autosomal recessive intellectual disability (MRT68), underscoring its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 460‑659) of human TRMT1; epitopes may differ among isoforms or across species—validate in the relevant sample system if working with non‑human or alternatively spliced targets.
• For Western blot, a band near 72 kDa is expected; consider using whole‑cell lysates from human (e.g., HeLa, HEK293) or mouse tissues, and confirm with appropriate loading controls.
• Due to mitochondrial/nuclear localization, subcellular fractionation can help enrich the target and improve detection.
• For ELISA, titrate the antibody to determine the optimal working dilution, as reactivity may vary with antigen presentation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this TRMT1 polyclonal antibody for tRNA modification and redox biology research. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare‑target sourcing, we offer a seamless procurement experience that bridges discovery and clinical application. Partner with us to streamline your assay development with reliable, high‑quality immunological reagents.