Anti-TERT Polyclonal Antibody for WB, ELISA - O14746

Core Product Specifications and Parameters

Biological Background: TERT Function and Localization

• Telomerase reverse transcriptase (TERT) is the catalytic subunit of the telomerase holoenzyme complex, a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes.
• Its primary function is to elongate telomeres by reverse transcription, adding the 6-nucleotide telomeric repeat sequence 5'-TTAGGG-3' to chromosome ends, using an internal RNA template within the telomerase RNA component.
• Telomerase activity is high in progenitor and cancer cells but low or absent in normal somatic cells; TERT plays critical roles in aging and antiapoptosis.
• TERT localizes to multiple subcellular compartments including the nucleus (nucleolus, nucleoplasm), chromosome telomeres, cytoplasm, and PML bodies.
• The protein undergoes alternative splicing and is subject to phosphorylation (Phosphoprotein) and ubiquitin-like conjugation (Ubl conjugation).
• Tissue-specific expression studies indicate high expression in thymocyte subpopulations, intermediate in tonsil T-lymphocytes, and low to undetectable in peripheral blood T-lymphocytes.
• Mutations in TERT are associated with dyskeratosis congenita and other telomere biology disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1000–1100 of human TERT; verify the epitope accessibility in your experimental context, especially in native versus denaturing conditions.
• For Western blotting, consider using nuclear-enriched lysates from cell lines with known telomerase activity (e.g., HeLa, 293T) to enhance detection, as TERT expression is often low in normal somatic cells.
• The antibody may cross-react with rat TERT; if using rat samples, validate performance in your specific tissue or cell type.
• For ELISA applications, optimize coating conditions and antibody concentration, and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide comprehensive IVD raw material procurement solutions, including validated antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This anti-TERT polyclonal antibody is part of our expanding portfolio for telomere biology and cancer research, and our team can assist with matching complementary reagents such as antibody pairs or recombinant proteins to accelerate your assay development pipeline.