Anti-TAT Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P17735

Core Product Specifications and Parameters

Biological Background: TAT Function and Localization

• TAT encodes tyrosine aminotransferase, a key enzyme in the catabolic pathway of tyrosine.
• Catalyzes the conversion of tyrosine to p-hydroxyphenylpyruvate, utilizing pyridoxal phosphate as a cofactor.
• In vitro, can also catalyze the reverse reaction with glutamic acid and 2-oxoglutarate.
• Exhibits significantly lower affinity and transaminase activity towards phenylalanine.
• Associated with disease variants linked to intellectual disability and palmoplantar keratoderma.
• Subject to post-translational modifications including acetylation and phosphorylation.
• Belongs to the aminotransferase class of transferases, critical for amino acid metabolism.
• The mature protein has a molecular weight of approximately 50 kDa (50,399 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-270 of human TAT; consider epitope-specific validation if targeting specific isoforms or modified forms.
• In Western blot (WB), expect a band near 50 kDa; use appropriate molecular weight markers and loading controls.
• For immunohistochemistry (IHC-P), validate antibody reactivity in relevant tissue sections and optimize antigen retrieval conditions.
• In ELISA applications, determine optimal coating and detection concentrations empirically.
• Cross-reactivity with mouse and rat TAT is reported; verify performance in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a comprehensive one-stop sourcing partner for IVD raw materials, bridging the gap between concept and clinical application for diagnostic manufacturers and research labs. For tyrosine metabolism and TAT-related studies, we offer a full suite of validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our integrated supply capabilities help streamline your procurement, ensuring reliable access to high-quality reagents to accelerate your assay development.