Anti-SOCS7 Monoclonal Antibody for WB, ELISA - O14512

Core Product Specifications and Parameters

Biological Background: SOCS7 Function and Localization

• As part of an ECS (CRL5) complex, SOCS7 recognizes phosphorylated substrates via its SH2 domain and mediates their ubiquitination and proteasomal degradation, targeting proteins such as DAB1 and IRS1. Related references: PMID:16127460
• The ECS(SOCS7) complex regulates reelin signaling by promoting ubiquitination and degradation of phosphorylated DAB1 in the developing cerebral cortex, which is essential for proper neuron positioning.
• SOCS7 functions in insulin signaling by targeting IRS1 for ubiquitination and subsequent proteasomal degradation, thereby modulating glucose homeostasis. Related references: PMID:16127460
• SOCS7 inhibits prolactin, growth hormone, and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their DNA-binding activity. Related references: PMID:15677474
• SOCS7 localizes to the cytoplasm, nucleus, and cell membrane, consistent with its roles in signal transduction and ubiquitination.
• SOCS7 is expressed in the brain, leukocytes, fetal lung fibroblasts, and fetal brain. Related references: PMID:9344857
• SOCS7 is characterized by an SH2 domain and is involved in the ubiquitin conjugation pathway; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human SOCS7. For Western blot, expect a band around 63 kDa in human cell lysates; validate in relevant human cell lines such as those derived from brain or leukocytes.
• For ELISA applications, consider using this antibody as a capture or detection reagent, but perform thorough optimization with recombinant SOCS7 protein and appropriate controls.
• Due to the monoclonal nature, lot-to-lot consistency should be high; however, always titrate the antibody in your own experimental system to determine optimal working concentrations.

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CamelBio offers diagnostic developers, research labs, and manufacturers a one-stop IVD raw-material sourcing solution, featuring high-quality monoclonal antibodies like this anti-SOCS7 clone for ubiquitination and cytokine signaling studies. We provide validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development from concept to clinic. Contact us to explore how our integrated supply chain can accelerate your project timelines.