Anti-SNRK Polyclonal Antibody for WB and ELISA - Q9NRH2

Core Product Specifications and Parameters

Biological Background: SNRK Function and Localization

• SNRK (SNF-related serine/threonine-protein kinase, alias HSNFRK) is a member of the SNF1/AMPK family of kinases.
• It may play a role in hematopoietic cell proliferation or differentiation, and is a potential mediator of neuronal apoptosis.
• The protein is located in the nucleus.
• Tissue expression is primarily in hematopoietic progenitor cells and leukemic cell lines, with weak expression in testis.
• Alternative splicing generates multiple isoforms, and the protein undergoes methylation and phosphorylation.
• Key features include ATP-binding, magnesium/metal-binding, nucleotide-binding, and serine/threonine-protein kinase activity.

Experimental Guidance and Technical Tips

• For Western blotting, consider using lysates from hematopoietic cell lines (e.g., K562, Jurkat) or neuronal models to evaluate SNRK expression. Pre-test at multiple dilutions to optimize signal-to-noise.
• The immunogen corresponds to amino acids 140-240, a region that may be conserved in mouse; verify cross-reactivity in mouse tissues.
• In ELISA, use a recombinant SNRK protein standard to establish assay conditions.
• Note the predicted molecular weight of ~84 kDa; ensure adequate gel electrophoresis and transfer conditions for high-molecular-weight proteins.
• As a polyclonal antibody, it may detect multiple isoforms; confirm specificity via siRNA knockdown or overexpression controls if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to IVD raw materials, supporting diagnostic manufacturers from concept to clinic. This SNRK polyclonal antibody is part of our extensive catalog of validated antibodies for hematology and neuroscience research. We also offer bulk ancillary reagents and custom sourcing for rare targets to streamline your assay development.