Anti-RNH1 Polyclonal Antibody for WB, IF/ICC, ELISA - P13489

Core Product Specifications and Parameters

Biological Background: RNH1 Function and Localization

• Ribonuclease inhibitor (RNH1), also known as placental ribonuclease inhibitor or RAI, is a ubiquitously expressed cytoplasmic protein that belongs to the leucine-rich repeat (LRR) family.
• Functions as a potent inhibitor of pancreatic-type ribonucleases, including RNASE1, RNASE2, and angiogenin (ANG), through tight binding that blocks their catalytic activity. Related references: PMID:12578357 PMID:14515218 PMID:3219362
• Plays a critical role in redox homeostasis by modulating intracellular RNase activity in response to oxidative stress. Related references: PMID:17292889
• Constitutively inhibits the cytotoxic tRNA ribonuclease activity of ANG in the cytoplasm under non-stress conditions, preserving translational integrity. Related references: PMID:23843625 PMID:32510170
• Upon cellular stress, RNH1 translocates to the nucleus, releasing cytoplasmic ANG inhibition while suppressing nuclear angiogenic activity of ANG, implicating RNH1 in stress-responsive signaling. Related references: PMID:23843625
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or subcellular localization; disease-associated variants have been reported, underscoring its physiological importance.
• RNH1 contains multiple leucine-rich repeats that form a horseshoe-shaped structure, providing an extended binding interface for diverse RNases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 202–461 of human RNH1 (NP_002930.2), encompassing critical LRR domains that are highly conserved across human, mouse, and rat; therefore, consider validating cross-reactivity in your specific sample system.
• For Western blotting, a band of approximately 50 kDa is expected; include appropriate positive and negative controls and optimize primary antibody dilution.
• For immunofluorescence/immunocytochemistry, fixation and permeabilization conditions may influence detection; we recommend testing both cytoplasmic and nuclear staining patterns, particularly under stress conditions where RNH1 relocates.
• As an ELISA-suitable antibody, it may be used for quantification of RNH1 in cell lysates or serum; however, assay conditions must be established by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical support to diagnostic manufacturers and research laboratories. Our RNH1-related products, including validated polyclonal antibodies, monoclonal antibodies, and ancillary reagents, are designed to streamline your assay development from concept to clinic. Whether you require bulk quantities or rare‑target sourcing, CamelBio is your reliable partner for high‑performance reagents in the ribonuclease inhibitor research area.