Anti-RNGTT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O60942

Core Product Specifications and Parameters

Biological Background: RNGTT Function and Localization

• RNGTT encodes the bifunctional mRNA-capping enzyme, also known as HCAP1 or HCE.
• Catalyzes the first two steps of mRNA cap formation: RNA 5'-triphosphate monophosphatase activity removes the gamma-phosphate from nascent mRNA, and mRNA guanylyltransferase transfers GMP to the 5'-diphosphate terminus.
• Functions as both a hydrolase and a nucleotidyltransferase, forming a covalent enzyme-GMP intermediate during the guanylyltransferase step.
• Localized to the nucleus, consistent with its role in co-transcriptional mRNA processing.
• Alternative splicing produces multiple isoforms; isoform 1 and isoform 4 are expressed in various tissues including cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin, and muscle.
• Classified under keywords: mRNA capping, mRNA processing, multifunctional enzyme, nucleotide-binding, host-virus interaction, and proteomics identification.
• The molecular weight of the canonical isoform is approximately 68.6 kDa, consistent with observed bands around 69 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-260 of human RNGTT, corresponding to the N-terminal region containing the triphosphatase domain. For applications requiring detection of the full-length protein, verify that the epitope is accessible in your experimental conditions.
• Based on the provided applications, this antibody is suitable for western blot (predicted band ~69 kDa), immunofluorescence/immunocytochemistry (nuclear staining), and ELISA.
• Cross-reactivity is reported for human, mouse, and rat; validation in other species is recommended if needed.
• For ELISA, consider pairing with a suitable detection antibody or using the antibody as a capture reagent, but optimal dilutions should be empirically determined.
• Store the antibody as shipped (ice bag) and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing for diagnostic manufacturers and research labs. This anti-RNGTT rabbit polyclonal antibody supports studies on mRNA cap formation and gene expression regulation. Beyond primary antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—helping you accelerate assay development from concept to clinic.